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Monarch® Spin Plasmid Miniprep Kit

Catalog # Concentration Size List Price Quantity Your Price
T1110L 250 preps $399.00
$359.10
$299.25
T1110S 50 preps $99.00
$89.10
$74.25
Catalog # Size List Price Your Price
T1110L 250 preps $399.00
$359.10
$299.25
T1110S 50 preps $99.00
$89.10
$74.25
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • A new version of plasmid miniprep kit, featuring upgraded spin columns precision engineered for high performance
  • Elute in as little as 30 μl for highly concentrated DNA, with yields up to 20 μg
  • Prevent buffer retention and salt carry over with unique, optimized column design
  • Easy to follow workflow with colored buffer system to indicate completion of key steps
  • Reduce hands-on time with faster protocols and less spin time
  • Significantly less plastic by design in columns and kit compared to leading suppliers

T1110 Box

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    Monarch® Plasmid Miniprep Kit protocol
  • Monarch_MiniprepProtocol_VideoThumb
    Tips for using the Monarch® Plasmid Miniprep Kit

The Monarch Spin Plasmid Miniprep Kit offers a unique method for plasmid preparation and purification, enabling extraction of highly concentrated plasmid DNA from various bacterial strains, with yields up to 20 μg of high-quality DNA. This new version features upgraded spin columns precision engineered to uniquely allow for low elution, in as little as 30 μl, for highly concentrated DNA in downstream application such as sequencing, restriction digestion, transformation, transfection, and more. A colored buffer system helps indicate completion of key lysis and neutralization steps for an easy to follow workflow and shorter, faster protocols allow for reduced hands-on time and less spin time. Compared to leading suppliers, these kits offer significantly less plastic through uniquely designed columns and bottles with plastic reduction while still maintaining structural integrity for centrifugation or vacuum manifolds and other steps. The kit features Monarch spin columns that are manufactured using state of the art automation machinery for high quality and consistency.

Properties
Purification format Spin column for nucleic acid purification
Compatible methods Centrifugation or vacuum manifold
Intended Usage Plasmid extraction and preparation from various E.coli host strains and other microbial strains
Plasmid DNA Yield Up to 20 μg*
Culture volume 1-5 ml, not to exceed 15 OD units
Column Binding Capacity Up to 20 μg
Plasmid Size Up to 25 kb
Elution Volume ≥ 30 μl
DNA Purity A260/280 > 1.8 and A260/230 > 1.8
Protocol Time 9.5 - 12.5 minutes of spin and incubation time
Compatible Downstream Applications Restriction digestion and other enzymatic manipulations, transformation, transfection, DNA sequencing, PCR, labeling, cell-free protein synthesis, etc.
 * Yield depends on plasmid copy number, host strain, culture volume and growth conditions

 

FIGURE 1: Monarch Spin Plasmid Miniprep Kit consistently produces more concentrated plasmid DNA with equivalent or better yield and purity compared to the leading supplier

T1110 Concentration

DNA concentration, yield and purity are higher with Monarch Spin Plasmid Miniprep Kit than leading supplier’s kit, across different plasmids. Preps were performed according to recommended protocols using ~1 ml (OD600 = 3) aliquot for pUC19 and pHT01 and ~2 ml (OD600 = 6) aliquot for Rluc and pXba of the same overnight cultures. Concentrations of plasmid were measured using a Trinean DropSense 16.



FIGURE 2: Plasmid DNA purified using Monarch Spin Plasmid Miniprep Kit are high-quality and suitable for downstream applications

T1110 Quality

Corresponding gel data shows purified plasmid DNA from Monarch Spin Plasmid Miniprep Kit works as well as leading supplier’s kit for downstream applications such as restriction digests. Gel data shows three replicates of plasmid purifications and subsequent restriction digests. Preps were performed according to recommended protocols using ~1 ml (OD600 = 3) aliquot for pUC19 and pHT01 and ~2 ml (OD600 = 6) aliquot for Rluc and pXba of the same overnight cultures. 1 μg of each prep was digested with XmnI (NEB #R0194) for pUC19, RLuc and pXba, and EcoRI (NEB #R0101) for pHT01 to linearize the plasmid and digests were resolved on 1.2% w/v TBE gel.



FIGURE 3: Monarch Spin Plasmid Miniprep Kit can elute in as low as 30 μl for high yield

T1110 Yield

Various elution volumes used in Monarch Spin Plasmid Miniprep Kit can generate high yield, highly concentrated DNA. 2 ml of NEB 10-beta Competent E. coli (NEB #C3019) transformed with pUC19 plasmid (NEB #N3041) was used. Monarch Buffer EY was used to elute the plasmid with the volumes indicated. Concentrations of plasmid were measured using a Trinean DropSense 16.



FIGURE 4: Features of the Monarch Spin Column S2D for Plasmid and Nucleic Acid Purification 

T1110 Column Design

NEB® Monarch’s unique column design and membrane assembly allows high-quality, highly-concentrated nucleic acid purification with low elution volume, for downstream applications. The column is designed and made with significantly less plastic for a reduced environmental impact.



FIGURE 5: Overview of the Monarch Spin Plasmid Miniprep Kit workflow

T1110 Workflow

The Monarch Spin Plasmid Miniprep Kit features unique spin columns and optimized buffers based on alkaline lysis.



Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Monarch® Spin Columns S2D T1117-1 25 1 x 50 columns
  Monarch® Spin Collection Tubes T2118-1 25 1 x 50 tubes
  Monarch® Buffer B1 T1111-1 25 1 x 12 ml
  Monarch® Buffer B2 T1112-1 25 1 x 11 ml
  Monarch® Buffer B3 T1113-1 25 1 x 22 ml
  Monarch® Buffer BZ T1114-1 25 1 x 8.4 ml
  Monarch® Buffer WZ T1115-1 25 1 x 5 ml
  Monarch® Buffer EY T1116-1 25 1 x 7 ml
  Monarch® RNase A T3018-1 -20 1 x 0.17 ml 20 mg/ml
  Monarch® Spin Columns S2D T1117-1 25 5 x 50 columns
  Monarch® Spin Collection Tubes T2118-1 25 5 x 50 tubes
  Monarch® Buffer B1 T1111-2 25 1 x 57 ml
  Monarch® Buffer B2 T1112-2 25 2 x 27 ml
  Monarch® Buffer B3 T1113-2 25 1 x 110 ml
  Monarch® Buffer BZ T1114-3 25 1 x 42 ml
  Monarch® Buffer WZ T1115-3 25 1 x 26 ml
  Monarch® Buffer EY T1116-2 25 1 x 25 ml
  Monarch® RNase A T3018-2 -20 1 x 0.5 ml 20 mg/ml
Features
  • High Performance: Achieve high yields (up to 20 μg) and high purity in the extraction and concentration of plasmid DNA without genomic DNA or RNA contamination.
  • High Concentration: Elute in as low as 30 μl, allowing for highly concentrated plasmid DNA.
  • Time Savings: 9.5- 12.5 minutes of spin and incubation time needed to complete the workflow.
  • Unique Design: The spin column features a unique design that enables elution in low volumes and minimizes buffer retention and contaminant carryover.
  • Optimized: The buffer system is optimized with color indicators to monitor the completion of each step.
  • Application Compatibility: Purified plasmid DNA is ready-to-use for restriction digestion and other enzymatic manipulations, transformation, transfection, DNA sequencing, PCR, labeling, cell-free protein synthesis, etc.

An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

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