Monarch® Plasmid Lysis Buffer (B2)

Catalog #	Size	List Price	Your Price
T1012L	54 ml

Monarch Plasmid Lysis Buffer (B2) is designed for use with the Monarch Plasmid Miniprep Kit (T1010S/L). The Lysis buffer is used to break open the cells under alkaline conditions in order to release the cellular components, including the plasmid DNA. For easy identification, the buffer is colored blue. This also helps to monitor the completion of the cell lysis step.

Properties & Usage

Companion Products

Protocols

Usage Guidelines

FAQs

Are plasmids recovered using the Monarch Plasmid Miniprep Kit endotoxin free?
I have used 5 ml of cell culture for plasmid isolation with the Monarch Plasmid Miniprep kit and I am obtaining low amounts of plasmid and/or contaminating gDNA. Why is this, and what are your suggestions to improve yield and purity?

Troubleshooting

Troubleshooting Guide for DNA Cleanup and Plasmid Purification

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

T1012L_v1

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

Monarch^® Plasmid Lysis Buffer (B2)

Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.