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SbfI-HF®

Catalog # Concentration Size List Price Quantity Your Price
R3642L 20000 units/ml 2500 units $497.00
$447.30
R3642S 20000 units/ml 500 units $120.00
$108.00
Catalog # Size List Price Your Price
R3642L 2500 units $497.00
$447.30
R3642S 500 units $120.00
$108.00
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

High-Fidelity (HF®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmart™ Buffer).  All HF-restriction enzymes come with Gel Loading Dye, Purple (6X).  Enjoy the enhanced performance and added value of our engineered enzymes at the same price as the native enzyme:

  • Engineered for improved performance
  • 100% activity in rCutSmart Buffer
  • Time-Saver™ qualified for digestion in 5-15 minutes
  • Reduced star activity
  • Supplied with 1 vial of Gel Loading Dye, Purple (6X)
  • Restriction Enzyme Cut Site: CCTGCA/GG
Featured Videos
View Video Library
  • Monarch_MiniprepProtocol_VideoThumb
    Reduce Star Activity with High-Fidelity Restriction Enzymes
  • Monarch_MiniprepProtocol_VideoThumb
    TIME-SAVER™ Protocol for Restriction Enzyme Digests
  • Monarch_MiniprepProtocol_VideoThumb
    NEB® TV Ep. 15 – Applications of Restriction Enzymes
  • Monarch_MiniprepProtocol_VideoThumb
    Restriction Enzyme Digest Protocol: Cutting Close to DNA End
  • Monarch_MiniprepProtocol_VideoThumb
    Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel
  • Monarch_MiniprepProtocol_VideoThumb
    Why is My Restriction Enzyme Not Cutting DNA?
  • Monarch_MiniprepProtocol_VideoThumb
    Restriction Enzyme Digest Problem: Too Many DNA Bands
  • Monarch_MiniprepProtocol_VideoThumb
    Double Digestion with NEBcloner
High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.

NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.

Restriction Enzyme Competitor Study: Nuclease Contamination


EcoRI, NotI, and BamHI from multiple suppliers were tested in reactions containing a fluorescent labeled single stranded, double stranded blunt, 3’overhang or 5’ overhang containing oligonucleotides. The percent degradation is determined by capillary electrophoresis and peak analysis. The resolution is at the single nucleotide level.
Product Source
An E. coli strain that carries the cloned and modified SbfI gene from Streptomycesspecies Bf-61 (S.K. Degtyarev).
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  SbfI-HF® R3642SVIAL -20 1 x 0.025 ml 20,000 units/ml
  rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X
  Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X
  SbfI-HF® R3642LVIAL -20 1 x 0.125 ml 20,000 units/ml
  rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X
  Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to digest 1μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Reaction Conditions

1X rCutSmart™ Buffer
Incubate at 37°C

1X rCutSmart™ Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Activity in NEBuffers

NEBufferâ„¢ r1.1: 50%
NEBufferâ„¢ r2.1: 25%
NEBufferâ„¢ r3.1: <10%>
rCutSmartâ„¢ Buffer: 100%

Diluent Compatibility

Storage Buffer

10 mM Tris-HCl
200 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml Recombinant Albumin
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

80°C for 20 minutes

Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive

Isoschizomers

SbfI
SdaI
Sse8387I



Companion Products
Materials Sold Separately
Notes
  • Based on the stability of the enzyme in the reaction, incubations longer than 1 hr will not result in improved digestion, unless additional enzyme is added. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.
  • Not sensitive to CpG, dcm, or dam methylation.
FAQs
Troubleshooting
Citations
Additional Citations
  • Hou Y, Nowak MD, Mirré V, Bjorå CS, Brochmann C, Popp M. (2016) RAD-seq data point to a northern origin of the arctic-alpine genus Cassiope (Ericaceae). Mol Phylogenet Evol Feb;95,PubMedID: 152-60
  • Kanamori A, Sugita Y, Yuasa Y, Suzuki T, Kawamura K, Uno Y, Kamimura K, Matsuda Y, Wilson CA, Amores A, Postlethwait JH, Suga K, Sakakura Y. (2016) A Genetic Map for the Only Self-Fertilizing Vertebrate. G3 (Bethesda) Apr 7;6(4), 1095-106.
  • Kemppainen P, Knight CG, Sarma DK, Hlaing T, Prakash A, Maung Maung YN, Somboon P, Mahanta J, Walton C. (2015) Linkage disequilibrium network analysis (LDna) gives a global view of chromosomal inversions, local adaptation and geographic structure. Mol Ecol Resour Sep;15(5), 1031-45.
  • Demos TC, Kerbis Peterhans JC, Joseph TA, Robinson JD, Agwanda B, Hickerson MJ. (2015) Comparative Population Genomics of African Montane Forest Mammals Support Population Persistence across a Climatic Gradient and Quaternary Climatic Cycles. PLoS One Sep 22;10(9), e0131800.
  • Lescak EA, Bassham SL, Catchen J, Gelmond O, Sherbick ML, von Hippel FA, Cresko WA. (2015) Evolution of stickleback in 50 years on earthquake-uplifted islands. Proc Natl Acad Sci U S A Dec 29;112(52), E7204-12.
  • Herrera S, Watanabe H, Shank TM. (2015) Evolutionary and biogeographical patterns of barnacles from deep-sea hydrothermal vents. Mol Ecol Feb;24(3), 673-89.
  • Hou Y, Nowak MD, Mirré V, Bjorå CS, Brochmann C, Popp M. (2015) Thousands of RAD-seq Loci Fully Resolve the Phylogeny of the Highly Disjunct Arctic-Alpine Genus Diapensia (Diapensiaceae). PLoS One Oct 8;10(10), e0140175.
  • Gamble T, Coryell J, Ezaz T, Lynch J, Scantlebury DP, Zarkower D. (2015) Restriction Site-Associated DNA Sequencing (RAD-seq) Reveals an Extraordinary Number of Transitions among Gecko Sex-Determining Systems. Mol Biol Evol May;32(5), 1296-309.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Change Notifications
SbfI-HF has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10187676. All subsequent (higher number) lots will contain rAlbumin. For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin).
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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