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SbfI

Catalog # Concentration Size List Price Quantity Your Price
R0642L 10000 units/ml 2500 units
Please Inquire
R0642S 10000 units/ml 500 units
Please Inquire
Catalog # Size List Price Your Price
R0642L 2500 units
R0642S 500 units
Please Inquire
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • Time-Saver™ qualified for digestion in 5-15 minutes
  • 100% activity in rCutSmart™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
  • High Fidelity (HF®) version available (NEB #R3642) supplied with rCutSmart Buffer
  • Restriction Enzyme Cut Site: CCTGCA/GG
Featured Videos
View Video Library
  • Monarch_MiniprepProtocol_VideoThumb
    Reduce Star Activity with High-Fidelity Restriction Enzymes
  • Monarch_MiniprepProtocol_VideoThumb
    TIME-SAVER™ Protocol for Restriction Enzyme Digests
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    NEB® TV Ep. 15 – Applications of Restriction Enzymes
  • Monarch_MiniprepProtocol_VideoThumb
    Restriction Enzyme Digest Protocol: Cutting Close to DNA End
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    Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel
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    Why is My Restriction Enzyme Not Cutting DNA?
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    Restriction Enzyme Digest Problem: Too Many DNA Bands
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    Double Digestion with NEBcloner

SbfI has an High Fidelity version, SbfI-HF® (NEB #R3642)

High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.

Product Source
An E. coli strain that carries the SbfI gene from Streptomyces species Bf-61 (S.K. Degtyarev).

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.

Reaction Conditions

1X rCutSmart™ Buffer
Incubate at 37°C

1X rCutSmart™ Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Activity in NEBuffers

NEBufferâ„¢ r1.1: 50%
NEBufferâ„¢ r2.1: 25%
NEBufferâ„¢ r3.1: <10%>
rCutSmartâ„¢ Buffer: 100%

Diluent Compatibility

Storage Buffer

10 mM Tris-HCl
50 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

80°C for 20 minutes

Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive



Companion Products
Materials Sold Separately
Notes
  • SbfI is an isoschizomer of Sse8387I.
  • Not sensitive to CpG, dcm, or dam methylation.
Citations
Additional Citations
  • Qiu S, Bergero R, Guirao-Rico S, Campos JL, Cezard T, Gharbi K, Charlesworth D. (2016) RAD mapping reveals an evolving, polymorphic and fuzzy boundary of a plant pseudoautosomal region. Mol Ecol Jan;25(1), 414-30.
  • Qiu S, Bergero R, Guirao-Rico S, Campos JL, Cezard T, Gharbi K, Charlesworth D. (2016) RAD mapping reveals an evolving polymorphic and fuzzy boundary of a plant pseudoautosomal region Mol Ecol Jan;25(1), 414-30.
  • Díaz-Arce N, Arrizabalaga H, Murua H, Irigoien X, Rodríguez-Ezpeleta N. (2016) RAD-seq derived genome-wide nuclear markers resolve the phylogeny of tunas. Mol Phylogenet Evol Sep;102, 202-7.
  • Araneda C, Larraín MA, Hecht B, Narum S. (2016) Adaptive genetic variation distinguishes Chilean blue mussels (Mytilus chilensis) from different marine environments. Ecol Evol Apr 26,
  • Rutledge LY, Devillard S, Boone JQ, Hohenlohe PA, White BN. (2015) RAD sequencing and genomic simulations resolve hybrid origins within North American Canis. Bio Lett Jul;11(7)., pii: 20150303. .
  • Liu S, Vallejo RL, Palti Y, Gao G, Marancik DP, Hernandez AG, Wiens GD. (2015) Identification of single nucleotide polymorphism markers associated with bacterial cold water disease resistance and spleen size in rainbow trout. Front Genet Sep 24;6:298.
  • Mathers TC, Hammond RL, Jenner RA, Hänfling B, Atkins J, Gómez A. (2015) Transition in sexual system and sex chromosome evolution in the tadpole shrimp Triops cancriformis. Heredity (Edinb) Jul;115(1), 37-46.
  • Zhou Z, Liu S, Dong Y, Gao S, Chen Z, Jiang J, Yang A, Sun H, Guan X, Jiang B, Wang B. (2015) High-Density Genetic Mapping with Interspecific Hybrids of Two Sea Urchins, Strongylocentrotus nudus and S. intermedius, by RAD Sequencing. PLoS One Sep 23;10(9),
  • Longo G, Bernardi G. (2015) The evolutionary history of the embiotocid surfperch radiation based on genome-wide RAD sequence data. Mol Phylogenet Evol Jul;88, 55-63.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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