Protocols

1. Optimizing Restriction Endonuclease Reactions
2. Restriction Digest Protocol
3. Double Digest Protocol with Standard Restriction Enzymes

Usage Guidelines

• Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
• Activity of Restriction Enzymes in PCR Buffers
• Cleavage Close to the End of DNA Fragments
• Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
• Double Digests
• Heat Inactivation
• Restriction Endonucleases - Survival in a Reaction
• Restriction Enzyme Diluent Buffer Compatibility
• Restriction Enzyme Tips
• Single Letter Codes
• Star Activity

Selection Charts

• Alphabetized List of Recognition Sequences
• Compatible Cohesive Ends and Generation of New Restriction Sites
• Dam-Dcm and CpG Methylation
• Frequencies of Restriction Sites
• Isoelectric Points (pI) for Restriction Enzymes
• Isoschizomers
• NEB Diluent and Buffer Table
• Time-Saver™ Qualified Enzymes

Web Tools

• DNA Sequences and Maps Tool
• Double Digest Finder
• Enzyme Finder
• NEBcutter™ v3.0
• NEBioCalculator®
• REBASE®

FAQs

• Is SbfI a Time-Saver™ Qualified enzyme?
• Is SbfI activity sensitive to dam, dcm or mammalian CpG methylation?
• How many base pairs should be added at the end of a PCR primer next to the SbfI recognition site to guarantee that SbfI will cut properly?
• Does SbfI have any neoschizomers?
• Is there a High Fidelity version of SbfI?
• Does SbfI exhibit star activity?
• Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
• I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
• Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

Citations

Additional Citations

• Qiu S, Bergero R, Guirao-Rico S, Campos JL, Cezard T, Gharbi K, Charlesworth D. (2016) RAD mapping reveals an evolving, polymorphic and fuzzy boundary of a plant pseudoautosomal region. Mol Ecol Jan;25(1), 414-30.
• Qiu S, Bergero R, Guirao-Rico S, Campos JL, Cezard T, Gharbi K, Charlesworth D. (2016) RAD mapping reveals an evolving polymorphic and fuzzy boundary of a plant pseudoautosomal region Mol Ecol Jan;25(1), 414-30.
• Díaz-Arce N, Arrizabalaga H, Murua H, Irigoien X, Rodríguez-Ezpeleta N. (2016) RAD-seq derived genome-wide nuclear markers resolve the phylogeny of tunas. Mol Phylogenet Evol Sep;102, 202-7.
• Araneda C, Larraín MA, Hecht B, Narum S. (2016) Adaptive genetic variation distinguishes Chilean blue mussels (Mytilus chilensis) from different marine environments. Ecol Evol Apr 26,
• Rutledge LY, Devillard S, Boone JQ, Hohenlohe PA, White BN. (2015) RAD sequencing and genomic simulations resolve hybrid origins within North American Canis. Bio Lett Jul;11(7)., pii: 20150303. .
• Liu S, Vallejo RL, Palti Y, Gao G, Marancik DP, Hernandez AG, Wiens GD. (2015) Identification of single nucleotide polymorphism markers associated with bacterial cold water disease resistance and spleen size in rainbow trout. Front Genet Sep 24;6:298.
• Mathers TC, Hammond RL, Jenner RA, Hänfling B, Atkins J, Gómez A. (2015) Transition in sexual system and sex chromosome evolution in the tadpole shrimp Triops cancriformis. Heredity (Edinb) Jul;115(1), 37-46.
• Zhou Z, Liu S, Dong Y, Gao S, Chen Z, Jiang J, Yang A, Sun H, Guan X, Jiang B, Wang B. (2015) High-Density Genetic Mapping with Interspecific Hybrids of Two Sea Urchins, Strongylocentrotus nudus and S. intermedius, by RAD Sequencing. PLoS One Sep 23;10(9),
• Longo G, Bernardi G. (2015) The evolutionary history of the embiotocid surfperch radiation based on genome-wide RAD sequence data. Mol Phylogenet Evol Jul;88, 55-63.

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• R0642S_L_v1

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• R0642S_L_v1_0471211
• R0642S_L_v1_0471302
• R0642S_L_v1_0471303
• R0642S_L_v1_0471309
• R0642S_L_v1_0471402
• R0642S_L_v1_0471408
• R0642S_L_v1_0471501
• R0642S_L_v1_0471507
• R0642S_L_v1_0471512
• R0642S_L_v1_0471608
• R0642S_L_v1_0471701
• R0642S_L_v1_0471708
• R0642S_L_v1_0471804
• R0642L_v1_10008626
• R0642L_v1_10025850
• R0642S_v1_10025852
• R0642S_v1_10041079
• R0642L_v1_10046527
• R0642L_v1_10056080
• R0642S_v1_10057661
• R0642S_v1_10078413
• R0642S_v1_10091207
• R0642L_v1_10084456
• R0642S_v1_10093315
• R0642L_v1_10100850
• R0642S_v1_10117588
• R0642L_v1_10114608

Safety Data Sheets

• SbfI

Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.