New England Biolabs Canada
Expand menu
Sign In or Sign UpMy NEB

DpnII

Catalog # Concentration Size List Price Quantity Your Price
R0543L 10000 units/ml 5000 units $480.00
$432.00
R0543M 50000 units/ml 5000 units $480.00
$432.00
R0543S 10000 units/ml 1000 units $115.00
$103.50
R0543T 50000 units/ml 1000 units $115.00
$103.50
Catalog # Size List Price Your Price
R0543L 5000 units $480.00
$432.00
R0543M 5000 units $480.00
$432.00
R0543S 1000 units $115.00
$103.50
R0543T 1000 units $115.00
$103.50
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • Time-Saver™ qualified for digestion in 5-15 minutes
  • Restriction Enzyme Cut Site: /GATC
Featured Videos
View Video Library
  • Monarch_MiniprepProtocol_VideoThumb
    Reduce Star Activity with High-Fidelity Restriction Enzymes
  • Monarch_MiniprepProtocol_VideoThumb
    TIME-SAVER™ Protocol for Restriction Enzyme Digests
  • Monarch_MiniprepProtocol_VideoThumb
    NEB® TV Ep. 15 – Applications of Restriction Enzymes
  • Monarch_MiniprepProtocol_VideoThumb
    Restriction Enzyme Digest Protocol: Cutting Close to DNA End
  • Monarch_MiniprepProtocol_VideoThumb
    Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel
  • Monarch_MiniprepProtocol_VideoThumb
    Why is My Restriction Enzyme Not Cutting DNA?
  • Monarch_MiniprepProtocol_VideoThumb
    Restriction Enzyme Digest Problem: Too Many DNA Bands
  • Monarch_MiniprepProtocol_VideoThumb
    Double Digestion with NEBcloner
Product Source
An E. coli strain that carries the DpnII gene from Diplococcus pneumoniae G41 (S. Lacks).
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  DpnII R0543SVIAL -20 1 x 0.1 ml 10,000 units/ml
  NEBuffer™ DpnII B0543SVIAL -20 1 x 1.25 ml 10 X
  Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X
  DpnII R0543TVIAL -20 1 x 0.02 ml 50,000 units/ml
  NEBuffer™ DpnII B0543SVIAL -20 1 x 1.25 ml 10 X
  Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X
  DpnII R0543LVIAL -20 1 x 0.5 ml 10,000 units/ml
  NEBuffer™ DpnII B0543SVIAL -20 1 x 1.25 ml 10 X
  Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X
  DpnII R0543MVIAL -20 1 x 0.1 ml 50,000 units/ml
  NEBuffer™ DpnII B0543SVIAL -20 1 x 1.25 ml 10 X
  Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (dam-) in 1 hour at 37°C in a total reaction volume of 50 µl.

Reaction Conditions

1X NEBuffer™ DpnII
Incubate at 37°C

1X NEBuffer™ DpnII
50 mM Bis-Tris-HCl
100 mM NaCl
10 mM MgCl2
0.1 mM DTT
(pH 6 @ 25°C)

Activity in NEBuffers

NEBufferâ„¢ r1.1: 25%
NEBufferâ„¢ r2.1: 25%
NEBufferâ„¢ r3.1: 100%
rCutSmartâ„¢ Buffer: 25%

Diluent Compatibility

Storage Buffer

10 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
500 µg/ml Recombinant Albumin
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

65°C for 20 minutes

Methylation Sensitivity

dam methylation: Blocked
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive



Companion Products
Notes
  • DpnII and Sau3AI are isoschizomers of MboI.
  • Cleaves to leave a 5´ GATC extension which can be efficiently ligated to DNA fragments generated by BamHI, BclI, BglII, MboI, Sau3AI and BstYI.
  • This enzyme is blocked by dam methylation. More information can be found at Dam-Dcm and CpG Methylation.
  • Will exhibit star activity in NEBuffer r3.1. We recommend the use of NEB DpnII Unique Buffer.
FAQs
Troubleshooting
Tech Tips
  • This enzyme will not cut plasmids isolated from most E.coli because it is blocked by dam methylation. For enzyme cleavage to occur, plasmids must be transformed and isolated from a dam-, dcm- strain of E.coli, such as NEB’s dam-/dcm- Competent E.coli (NEB #C2925).
Citations
Additional Citations
  • Hughes J.R., Roberts N., McGowan S., Hay D., Giannoulatou E., Lynch M., De Gobbi M., Taylor S., Gibbons R., Higgs D.R. (2014) Analysis of hundreds of cis-regulatory landscapes at high resolution in a single, high-throughput experiment Nat Genet 46 (2), 205-212.PubMedID: 24413732, DOI: doi:10.1038/ng.2871
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Change Notifications
DpnII has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10174818. All subsequent (higher number) lots will contain rAlbumin. For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin).
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Other Products You May Be Interested In
 Top