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Trypsin-ultra™, Mass Spectrometry Grade

Catalog # Concentration Size List Price Quantity Your Price
P8101S 100 µg $138.00
$124.20
Catalog # Size List Price Your Price
P8101S 100 µg $138.00
$124.20
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Trypsin-ultra, Mass Spectrometry Grade is a serine endopeptidase, which selectively cleaves peptide bonds C-terminal to lysine and arginine residues.  Trypsin-ultra cleaves at Lys-Pro and Arg-Pro bonds at a much slower rate than when Lys and Arg are N-terminal to other residues.

  • Analyze complex proteomes with minimal autolysis
  • Compatible for simultaneous co-digestion with PNGase F (NEB# P0709)
  • Compatible for simultaneous co-digestion with Endoproteinase LysC (NEB# P8109)
  • Suitable for both in-gel and solution digests
  • Supplied in dry format
  • Optimal activity and stability for up to 12 months

Trypsin-ultra™, Mass Spectrometry Grade is a serine endopeptidase. It selectively cleaves peptide bonds C-terminal to lysine and arginine residues (1). Trypsin-ultra is treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inactivate any remaining chymotryptic activity. It is modified by acetylation of the ε-amino groups of lysine residues to prevent autolysis. Trypsin-ultra (TPCKtreated) cleaves at Lys-Pro and Arg-Pro bonds at a much slower rate than other amino acid residues (2).

Digestion with Trypsin-ultra results in a high number of proteins identified, and sequence coverages as high as 90%

3-hour Trypsin-ultra digest of Pyrococcus furiosus
A 3-hour Trypsin-ultra digest of Pyrococcus furiosus using a 1:50 enzyme:substrate ratio, followed by ESI-MS, resulted in 418 proteins identified with individual protein sequence coverage as high as 90%.
Product Source
Isolated from bovine (Bos taurus) pancreas.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Trypsin-ultra™, Mass Spectrometry Grade P8101SVIAL -20 5 x 20 µg
  Trypsin-ultra, Reaction Buffer B8101SVIAL -20 1 x 1.5 ml 2 X
Application Features
  • Digestion of proteins for proteomic analysis by Mass Spectrometry
  • Protein and peptide identification

Properties & Usage

Reaction Conditions

1X Trypsin-ultra, Reaction Buffer
Incubate at 37°C

1X Trypsin-ultra, Reaction Buffer
50 mM Tris-HCl
20 mM CaCl2
(pH 8 @ 25°C)

Usage Concentration

100 ng/μl

Molecular Weight

Theoretical: 23675 daltons


Companion Products
Notes
  • Substrate must be in phosphate-free buffer to prevent calcium precipitation with both reconstituted enzyme and enzyme buffer.
  • Trypsin-ultra, Mass Spectrometry Grade is acetylated on multiple lysine residues. This protein appears as a single band on SDS-PAGE. This sequence is also available at www.neb.com.
  • Storage Conditions: Supplied in dry format from a sodium acetate and calcium chloride buffer. Store at -20°C.
  • Can be stored frozen in solution at -20°C for up to 2 weeks. A decrease in activity will occur if stored in solution. Use only freshly reconstituted protease for best results.
References
  • Northrop, J.H. and Kunitz, M. (1931). Isolation of protein crystals possessing tryptic activity. Science. 73,
  • Perona, J.J. and Craik, C.S. (1995). Structural basis of substrate specificity in the serine proteases. Protein Sci.. 4, 337-360.
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Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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