Deglycosylation in minutes for intact antibody analysis
Developed for proteomic applications, Rapid PNGase F (non-reducing format) enables complete and rapid deglycosylation, while preserving disulfide bonds. This facilitates high throughput proteomics applications and methods for antibody characterization by mass spectrometry, such as intact mass analysis. Rapid PNGase F (non-reducing format) combines the advantages of Rapid PNGase F (fast processing time), with non-reducing conditions, preserving quaternary structure.
1X Rapid PNGase F (non-reducing format) Buffer
Incubate at 50°C
20 mM Tris-HCl
50 mM NaCl
5 mM EDTA
pH 7.5 @ 25°C
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