PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F (Glycerol-free), Recombinant is a recombinant amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides.
Peptide: N-Glycosidase F, also known
as PNGase F, is a recombinant amidase which is
supplied glycerol free for optimal performance
in HPLC intensive methods. PNGase F cleaves
between the innermost GlcNAc and asparagine
residues of high mannose, hybrid, and complex
oligosaccharides from N-linked glycoproteins (1).
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
1X GlycoBuffer 2
Incubate at 37°C
1X GlycoBuffer 2
50 mM Sodium Phosphate
(pH 7.5 @ 25°C)
50 mM NaCl
20 mM Tris-HCl
5 mM EDTA
pH 7.5 @ 25°C
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