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Endoproteinase AspN

Catalog # Concentration Size List Price Quantity Your Price
P8104S 50 µg $154.00
$138.60
Catalog # Size List Price Your Price
P8104S 50 µg $154.00
$138.60
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Endoproteinase AspN (flavastacin) is a zinc metalloendopeptidase which selectively cleaves protein and peptide bonds N-terminal to aspartic acid residues.

  • Free of glycerol and detergents which may interfere with mass spectrometry or liquid chromatography methods.
  • Supplied in dry format
  • Optimal activity and stability for up to 12 months
Endoproteinase AspN (flavastacin) is a zinc metalloendopeptidase which selectively cleaves peptide bonds N-terminal to aspartic acid residues(1).
Product Source
Purified from Flavobacterium menigosepticum.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Endoproteinase AspN P8104SVIAL -20 1 x 50 µg
  Endoproteinase AspN Reaction Buffer B8104SVIAL -20 1 x 1.5 ml 2 X

Properties & Usage

Reaction Conditions

1X Endoproteinase AspN Reaction Buffer
Incubate at 37°C

1X Endoproteinase AspN Reaction Buffer
50 mM Tris-HCl
2.5 mM ZnSO4
(pH 8 @ 25°C)

Usage Concentration

100 ng/μl

Molecular Weight

Theoretical: 40089.9 daltons


Companion Products
Notes
  • Storage Conditions: Supplied in dry format. Can be stored frozen in solution at -20°C for up to 2 weeks. A decrease in activity will occur if stored in solution. Use only freshly reconstituted protease for best results.
  • Endoproteinase AspN contains a O-linked carbohydrate on the protein. The protein appears as a single band by SDS-PAGE analysis.
  • Aspartic acid residues are strongly favored by Endoproteinase AspN in all buffer conditions we have examined (Tris-HCl, ammonium bicarbonate and potassium phosphate) (2).
References
  • Tarentino A.L. Flavastacin (2004). In Handbook of Proteolytic Enzymes. 2nd Ed., 631-632, Elsevier, London.
  • Tarentino, A.L. et al. (1995). Arch biochem biophys . 319, 281-285.
  • Grimwood, B.G., Plummer, T.H. and Tarentino, A.L. (1994). Arch Biochem Biophys . 311, 127-132.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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