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yDcpS

Catalog # Concentration Size List Price Quantity Your Price
M0463S 200000 units/ml 4000 units $429.00
$386.10
Catalog # Size List Price Your Price
M0463S 4000 units $429.00
$386.10
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

yDcpS is a decapping enzyme that hydrolyzes m7G capped mRNA, producing diphosphorylated 5′ ends. yDcpS can decap mRNAs of various lengths and the diphosphorylated 5′ end can be recapped directly with Vaccinia Capping Enzyme.

  • Provided at high enzyme concentration to facilitate complete decapping
  • Active on capped RNA of various sizes (tested on in vitro capped transcripts from 25 nt to 1400 nt long)
  • Decapped RNA 5′ ends can be recapped with Vaccinia Capping System (NEB #M2080)
  • Supports tagging of mRNA with desthiobiotin-labeled GTP
yDcpS is the scavenging decapping enzyme from S. cerevisiae that hydrolyzes the phosphodiester bond between the gamma and beta phosphates of m7G-capped mRNA, producing diphosphorylated 5′ ends and m7GMP.1,2 yDcpS is capable of decapping mRNAs of various lengths, decaps Cap0 and Cap1 with equal efficiency, and the diphosphorylated 5′ end it produces is suitable for recapping with Vaccinia Capping Enzyme.3,4
Product Source
A His-tagged engineered variant of E. coli that carries the S. cerevisiae gene DCS1 (encoding yDcpS) on a plasmid.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  yDcpS M0463SVIAL -20 1 x 0.02 ml 200,000 units/ml
  yDcpS Reaction Buffer B0463AVIAL -20 1 x 1 ml 10 X
Features
  • mRNA decapping enabling recapping with tagged-GTP analogs
  • Biotinylation of 5′ ends of primary transcripts
  • Recappable-seq
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Companion Products
References
  • Wang, X. and Kiledjian, M. (2001). Cell. 107 (6), 751-62.
  • Liu, H., Rodgers, N.D., Jiao, X., and Kiledjian, M. (2002). EMBO J.. 21(17), 4699-708.
  • Wulf MG, Buswell J, Chan SH, Dai N, Marks K, Martin ER, Tzertzinis G, Whipple JM, Corrêa IR Jr, Schildkraut I. (2019). The yeast scavenger decapping enzyme DcpS and its application for in vitro RNA recapping. Sci Rep. Jun 13; 9 (1), 8594. DOI: 10.1038/s41598-019-45083-5.
  • Bo Yan, George Tzertzinis, Ira Schildkraut, Laurence Ettwiller ReCappable Seq: Comprehensive Determination of Transcription Start Sites derived from all RNA polymerases. bioRxiv. 696559.
Citations
Additional Citations
  • Wulf, Madalee; Buswell, John; Chan, Siuhong; Dai, Nan; Marks, Katherine; Tzertzinis, George; Whipple, Joe; Correa, Ivan; Schildkraut, Ira; (2019) The yeast scavenger decapping enzyme DcpS and its application for in vitro RNA recapping Sci Rep 9 (1), 8594.PubMedID: 31197197, DOI: 10.1038/s41598-019-45083-5
Publications
  • Wulf, Madalee; Buswell, John; Chan, Siuhong; Dai, Nan; Marks, Katherine; Tzertzinis, George; Whipple, Joe; Correa, Ivan; Schildkraut, Ira; (2019). The yeast scavenger decapping enzyme DcpS and its application for in vitro RNA recapping Sci Rep. 9 (1), 8594.PubMedID: 31197197, DOI: 10.1038/s41598-019-45083-5
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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