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3´-Desthiobiotin-GTP

Catalog # Concentration Size List Price Quantity Your Price
N0761S 5 mM 0.50 µmol $534.00
$480.60
Catalog # Size List Price Your Price
N0761S 0.50 µmol $534.00
$480.60
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
3'-Desthiobiotin-GTP or 3'-Biotin GTP are guanosine triphosphate (GTP) analogs which are modified at their 3’ position with desthiobiotin or biotin, respectively. When used with the Vaccinia Capping System, (NEB #M2080) these reagents enable affinity tagging of RNA triphosphate ends. Tagged RNAs are enriched by binding to Hydrophilic Streptavidin Magnetic Beads (NEB #S1421). Desthiobiotin-tagged RNAs can be eluted with free biotin. This approach is used in Cappable-seq, a method developed at NEB for directly enriching the 5’-ends of primary transcripts (1).
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Supplied in: Ultrapure Water as a Triethylammonium salt at pH 7.0
Molecular Formula: C31H52N11O19P3
Molecular Weight: 975.74 g/mol
Extinction Coefficient: λ260 = ~11,700 Lmol-1 cm-1
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  3´-Desthiobiotin-GTP N0761SVIAL -20 1 x 0.1 ml 5 mM

Properties & Usage



Companion Products
References
  • Ettwiller, L. et al. (2016). BMC Genomics. 17, 199.
Quality Control Assay
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Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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