3´-Biotin-GTP

Catalog #	Concentration	Size	List Price	Quantity	Your Price
N0760S	5 mM	0.50 µmol	$534.00		$480.60

Catalog #	Size	List Price	Your Price
N0760S	0.50 µmol	$534.00	$480.60

3'-Desthiobiotin-GTP or 3'-Biotin GTP are guanosine triphosphate (GTP) analogs which are modified at their 3’ position with desthiobiotin or biotin, respectively. When used with the Vaccinia Capping System, (NEB #M2080) these reagents enable affinity tagging of RNA triphosphate ends. Tagged RNAs are enriched by binding to Hydrophilic Streptavidin Magnetic Beads (NEB #S1421). Desthiobiotin-tagged RNAs can be eluted with free biotin. This approach is used in Cappable-seq, a method developed at NEB for directly enriching the 5’-ends of primary transcripts (1).

Supplied in: Ultrapure water as a Triethylammonium salt at pH 7.0
Molecular Formula: C₃₁H₅₀N₁₁O₁₉P₃S
Molecular Weight: 1005.78 g/mol
Extinction Coefficient: λ260 = ~11,700 Lmol-1 cm-1

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

N0760S -20

	3´-Biotin-GTP	N0760SVIAL	-20	1 x 0.1 ml	5 mM

Properties & Usage

References

Boutard, M. et al. (2016). Nature Communications. DOI: 7:13783
Ettwiller, L. et al. (2016). BMC Genomics. DOI: 17:199

Citations

See more details on Bioz

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

3´-Biotin-GTP

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