New England Biolabs provides a color-coded 10X NEBuffer (with BSA) with each restriction endonuclease to ensure optimal (100%) activity.
This NEBuffer is available for those enzymes requiring NEBuffers without albumin.
For the albumin containing formulation (which is included with NEB restriction enzymes), NEBuffer™ r3.1 (NEB #B6003).
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl2
1 mM DTT
pH 7.9@25°C
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