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EcoO109I

Catalog # Concentration Size List Price Quantity Your Price
R0503S 20000 units/ml 2000 units $108.00
$97.20
Catalog # Size List Price Your Price
R0503S 2000 units $108.00
$97.20
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • 100% activity in rCutSmart™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
  • Restriction Enzyme Cut Site: RG/GNCCY

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    Product Source
    An E. coli strain that carries the cloned EcoO109I gene from E. coli H709c (I. Orskov).
    Reagents Supplied

    The following reagents are supplied with this product:

    NEB # Component Name Component # Stored at (°C) Amount Concentration
      EcoO109I R0503SVIAL -20 1 x 0.1 ml 20,000 units/ml
      rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X

    Properties & Usage

    Unit Definition

    One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (Hind III digest) in 1 hour at 37°C in a total reaction volume of 50 µl.

    Reaction Conditions

    1X rCutSmart™ Buffer
    Incubate at 37°C

    1X rCutSmart™ Buffer
    50 mM Potassium Acetate
    20 mM Tris-acetate
    10 mM Magnesium Acetate
    100 µg/ml Recombinant Albumin
    (pH 7.9 @ 25°C)

    Activity in NEBuffers

    NEBufferâ„¢ r1.1: 50%
    NEBufferâ„¢ r2.1: 100%
    NEBufferâ„¢ r3.1: 50%
    rCutSmartâ„¢ Buffer: 100%

    Diluent Compatibility

    Storage Buffer

    10 mM Tris-HCl
    1 mM DTT
    0.1 mM EDTA
    200 µg/ml Recombinant Albumin
    50% Glycerol
    50 mM KCl
    pH 7.4 @ 25°C

    Heat Inactivation

    65°C for 20 minutes

    Methylation Sensitivity

    dam methylation: Not Sensitive
    dcm methylation: Blocked by Overlapping
    CpG Methylation: Not Sensitive



    Companion Products
    Materials Sold Separately
    Notes
    • Eco0109I is an isoschizomer of DraII.
    • This enzyme has shown to have lower activity on some supercoiled plasmids, with more than 1 unit required to digest 1 μg plasmid DNA. For complete digestion of 1 μg of plasmid DNA please follow our recommended digestion protocol.
    • Blocked by overlapping dcm methylation.
    Quality Control Assay
    Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
    Change Notifications
    EcoO109I has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10133985. All subsequent (higher number) lots will contain rAlbumin. For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin).
    Specifications
    The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
    Certificate of Analysis
    The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
    Safety Data Sheets
    Legal And Disclaimer

    Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

    This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

    New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more. 

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