SARS-CoV-2 LAMP Primer Mix (N/E)

Catalog #	Concentration	Size	List Price	Quantity	Your Price
S1883S	10 X	85 reactions				Please Inquire

Catalog #	Size	List Price	Your Price
S1883S	85 reactions

What is the impact of the latest SARS-CoV-2 variant on this primer set? View the Primer Monitor Tool (more info here) for up-to-date information on variants mapped against common primer sets
Provided at 10X concentration
Targets N and E gene regions of SARS-CoV-2 genome
Optimized mixture of 2 primer sets for enhanced speed and sensitivity of SARS-CoV-2 viral RNA detection
Component of the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (NEB #E2019)
Information on using this product to detect SARS-CoV-2 viral RNA using WarmStart LAMP reagents with UDG can be viewed here
Need assistance designing your own LAMP primers? Use the NEB LAMP Primer Design Tool
Learn more about LAMP and other isothermal amplification methods
A publication by members of the global LAMP (gLAMP) Consortium provides a comprehensive review of LAMP and its role in the COVID-19 pandemic

The SARS-CoV-2 LAMP Primer Mix (N/E) is a dual-target primer mix that recognizes the nucleocapsid (N) and envelope (E) regions of the SARS-CoV-2 genome. It contains 2 sets of LAMP primers (F3, B3, FIP, BIP, LF, LB) for the enhanced detection of SARS-CoV-2 nucleic acid.

SARS-CoV-2 LAMP Primer Sequences (5´→ 3´)

Gene E1 Primer Set	Sequence
E1-F3	TGAGTACGAACTTATGTACTCAT
E1-B3	TTCAGATTTTTAACACGAGAGT
E1-FIP	ACCACGAAAGCAAGAAAAAGAAGTTCGTTTCGGAAGAGACAG
E1-BIP	TTGCTAGTTACACTAGCCATCCTTAGGTTTTACAAGACTCACGT
E1-LF	CGCTATTAACTATTAACG
E1-LB	GCGCTTCGATTGTGTGCGT

Gene N2 Primer Set	Sequence
N2-F3	ACCAGGAACTAATCAGACAAG
N2-B3	GACTTGATCTTTGAAATTTGGATCT
N2-FIP	TTCCGAAGAACGCTGAAGCGGAACTGATTACAAACATTGGCC
N2-BIP	CGCATTGGCATGGAAGTCACAATTTGATGGCACCTGTGTA
N2-LF	GGGGGCAAATTGTGCAATTTG
N2-LB	CTTCGGGAACGTGGTTGACC

Features

Optimized mixture of 2 primer sets for enhanced speed and sensitivity of SARS-CoV-2 viral RNA detection
Information on using this product to detect SARS-CoV-2 viral RNA using WarmStart LAMP reagents with UDG can be viewed here
View the protocol for recommended reaction setup

An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Protocols

Overview of Reaction Setup (NEB #S1883)

Web Tools

NEB LAMP Primer Design Tool

FAQs

Quality Control Assay

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Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

SARS-CoV-2 LAMP Primer Mix (N/E)

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