Control LAMP Primer Mix (rActin)

Catalog # Concentration Size List Price Quantity Your Price
S0164S 10 X 50 reactions $152.00
$136.80
Catalog # Size List Price Your Price
S0164S 50 reactions $152.00
$136.80
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • Provided at 10X concentration
  • Optimized mix of 6 primers (F3, B3, FIP, BIP, LF, LB) targets actin RNA at an exon-exon junction
  • Can be used in a LAMP control reaction to verify assay and reagent performance
  • Internal control component in the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (NEB #E2019)
  • Information on using this product to detect SARS-CoV-2 viral RNA using WarmStart LAMP reagents with UDG can be viewed here
  • Need assistance designing your own LAMP primers? Use the NEB LAMP Primer Design Tool
  • Learn more about LAMP and other isothermal amplification methods
  • A publication by members of the global LAMP (gLAMP) Consortium provides a comprehensive review of LAMP and its role in the COVID-19 pandemic
 
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The Control LAMP Primer Mix (rActin) targets actin RNA at an exon-exon junction. It is a primer set (F3, B3, FIP, BIP, LF, LB) that amplifies rActin and can be used to confirm the activity of reagents, proper sample handling and the presence of human nucleic acid template in a LAMP reaction.

This product serves as an internal control primer set in the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (NEB #E2019).

Control LAMP Primer Sequences: rActin (5´→ 3´)

rActin Primer Set Sequence
ACTB-F3 AGTACCCCATCGAGCACG
ACTB-B3 AGCCTGGATAGCAACGTACA
ACTB-FIP GAGCCACACGCAGCTCATTGTATCACCAACTGGGACGACA
ACTB-BIP CTGAACCCCAAGGCCAACCGGCTGGGGTGTTGAAGGTC
ACTB-LF TGTGGTGCCAGATTTTCTCCA
ACTB-LB CGAGAAGATGACCCAGATCATGT

 

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Control LAMP Primer Mix (rActin) S0164AVIAL -20 1 x 0.14 ml 10 X
Features
  • Optimized mixture of 2 primer sets for enhanced speed and sensitivity of SARS-CoV-2 viral RNA detection
  • Information on using this product to detect SARS-CoV-2 viral RNA using WarmStart LAMP reagents with UDG can be viewed here
  • View the protocol for recommended reaction setup

 

An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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