The Control LAMP Primer Mix (rActin) targets actin RNA at an exon-exon junction. It is a primer set (F3, B3, FIP, BIP, LF, LB) that amplifies rActin and can be used to confirm the activity of reagents, proper sample handling and the presence of human nucleic acid template in a LAMP reaction.
This product serves as an internal control primer set in the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (NEB #E2019).
Control LAMP Primer Sequences: rActin (5´→ 3´)
rActin Primer Set | Sequence |
---|---|
ACTB-F3 | AGTACCCCATCGAGCACG |
ACTB-B3 | AGCCTGGATAGCAACGTACA |
ACTB-FIP | GAGCCACACGCAGCTCATTGTATCACCAACTGGGACGACA |
ACTB-BIP | CTGAACCCCAAGGCCAACCGGCTGGGGTGTTGAAGGTC |
ACTB-LF | TGTGGTGCCAGATTTTCTCCA |
ACTB-LB | CGAGAAGATGACCCAGATCATGT |
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