WarmStart® Colorimetric LAMP 2X Master Mix with UDG

Catalog #	Concentration	Size	List Price	Quantity	Your Price
M1804L	2 X	500 reactions	$1,685.00		$1,516.50
M1804S	2 X	100 reactions	$422.00		$379.80

Catalog #	Size	List Price	Your Price
M1804L	500 reactions	$1,685.00	$1,516.50
M1804S	100 reactions	$422.00	$379.80

WarmStart Colorimetric LAMP 2X Master Mix with UDG is an optimized formulation of Bst 2.0 WarmStart^® DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of LAMP and RT-LAMP reactions. The inclusion of dUTP and UDG in the master mix reduces the possibility of carryover contamination between reactions.

This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow (an overview of LAMP and primer design can be found in the Featured Videos section). This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15–40 minutes.

Negative reactions are indicated in pink and positive reactions are indicated by a change to yellow.

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

M1804S -20

	WarmStart^® Colorimetric LAMP 2X Master Mix with UDG	M1804SVIAL	-20	1 x 1.32 ml	2 X

M1804L -20

	WarmStart^® Colorimetric LAMP 2X Master Mix with UDG	M1804SVIAL	-20	5 x 1.32 ml	2 X

Application Features

LAMP amplification of RNA and DNA targets
Simple visual indication of amplification for easy detection and analysis
UDG and dUTP included in master mix for carryover prevention

An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Companion Products

Protocols

WarmStart Colorimetric LAMP 2X Master Mix with UDG Typical LAMP Protocol (NEB #M1804)

Selection Charts

DNA Polymerase Selection Chart

Web Tools

FAQs

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Change Notifications

Product is now supplied in Sarstedt vials (February 28, 2022).

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

M1804S_L_v2

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

WarmStart^® Colorimetric LAMP 2X Master Mix with UDG

Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased product to perform loop-mediated amplification (“LAMP”) and reverse transcription loop-mediated amplification (“RT-LAMP”) for research use only. LAMP is a patented technology belonging to Eiken Chemical Co., Ltd and any other use other than research may require a license from Eiken Chemical Co., Ltd.

Nucleic acid-based aptamers for use with thermophilic DNA polymerases and reverse transcriptases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. Commercial use of this product may require a license from New England Biolabs, Inc. For additional information or to inquire about commercial use, please contact busdev@neb.com.