PvuII

Catalog #	Concentration	Size	List Price	Your Price
R0151L	10000 units/ml	25000 units	$413.00	$371.70
R0151M	50000 units/ml	25000 units	$413.00	$371.70
R0151S	10000 units/ml	5000 units	$101.00	$90.90
R0151T	50000 units/ml	5000 units	$101.00	$90.90

Catalog #	Size	List Price	Your Price
R0151L	25000 units	$413.00	$371.70
R0151M	25000 units	$413.00	$371.70
R0151S	5000 units	$101.00	$90.90
R0151T	5000 units	$101.00	$90.90

PvuII has a High Fidelity version PvuII-HF^® (NEB #R3151).

High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.

PvuII crystals (Ira Schildkraut and Joseph Bonventre, New England Biolabs)

Product Source

An E.coli strain that carries the PvuII gene from Proteus vulgaris (ATCC 13315).

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

R0151S -20

	PvuII	R0151SVIAL	-20	1 x 0.5 ml	10,000 units/ml
	NEBuffer™ r3.1	B6003SVIAL	-20	1 x 1.25 ml	10 X

R0151T -20

	PvuII	R0151TVIAL	-20	1 x 0.1 ml	50,000 units/ml
	NEBuffer™ r3.1	B6003SVIAL	-20	1 x 1.25 ml	10 X

R0151L -20

	PvuII	R0151LVIAL	-20	2 x 1.25 ml	10,000 units/ml
	NEBuffer™ r3.1	B6003SVIAL	-20	1 x 1.25 ml	10 X

R0151M -20

	PvuII	R0151MVIAL	-20	1 x 0.5 ml	50,000 units/ml
	NEBuffer™ r3.1	B6003SVIAL	-20	1 x 1.25 ml	10 X

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.

Reaction Conditions

1X NEBuffer™ r3.1
Incubate at 37°C

1X NEBuffer™ r3.1
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl₂
100 Âµg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Activity in NEBuffers

NEBufferâ„¢ r1.1: 50%
NEBufferâ„¢ r2.1: 100%
NEBufferâ„¢ r3.1: 100%
rCutSmartâ„¢ Buffer: 100%

Diluent Compatibility

Diluent B

Storage Buffer

10 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
500 Âµg/ml Recombinant Albumin
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive

Isoschizomers

PvuII-HF

Companion Products

Materials Sold Separately

NEBuffer™ r3.1

Notes

May exhibit star activity in rCutSmart Buffer.
PvuII does not cut CAGm4CTG, while cutting at CAGm5CTG occurs at a significantly reduced rate.
For enzymes that cannot be heat-inactivated, we recommend using a column for cleanup (such as the Monarch® PCR & DNA Cleanup Kit), or running the reaction on an agarose gel and then extracting the DNA (we recommend Monarch Gel Extraction Kit), or performing a phenol/chloroform extraction.

Protocols

Usage Guidelines

Selection Charts

Web Tools

FAQs

Troubleshooting

Restriction Enzyme Troubleshooting Guide

Citations

See more details on Bioz

Additional Citations

Marlena Kisiala, Monika Kowalska, Michal Pastor, Henryk J Korza, Honorata Czapinska, Matthias Bochtler (2020) Restriction endonucleases that cleave RNA/DNA heteroduplexes bind dsDNA in A-like conformation Nucleic Acids Res Volume 48, Issue 12, 6954–6969. DOI: 10.1093/nar/gkaa403

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Change Notifications

PvuII has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10196883. All subsequent (higher number) lots will contain rAlbumin. For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin).

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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