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SspI

Catalog # Concentration Size List Price Quantity Your Price
R0132L 5000 units/ml 5000 units
Please Inquire
R0132M 25000 units/ml 5000 units
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R0132S 5000 units/ml 1000 units
Please Inquire
Catalog # Size List Price Your Price
R0132L 5000 units
R0132M 5000 units
R0132S 1000 units
Please Inquire
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
SspI has a High Fidelity version SspI-HF® (NEB #R3132).

High Fidelity (HF®) Restriction Enzymes have 100% activity in CutSmart™ Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver™ qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
Product Source
An E. coli strain that carries the SspI gene from Sphaerotilus species (ATCC 13925).
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  SspI R0132SVIAL -20 1 x 0.2 ml 5,000 units/ml
  NEBuffer™ EcoRI/SspI B0101SVIAL -20 1 x 1.25 ml 10 X
  SspI R0132LVIAL -20 1 x 1 ml 5,000 units/ml
  NEBuffer™ EcoRI/SspI B0101SVIAL -20 1 x 1.25 ml 10 X
  SspI R0132MVIAL -20 1 x 0.2 ml 25,000 units/ml
  NEBuffer™ EcoRI/SspI B0101SVIAL -20 1 x 1.25 ml 10 X

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.

Reaction Conditions

1X NEBuffer™ EcoRI/SspI
Incubate at 37°C

1X NEBuffer™ EcoRI/SspI
100 mM Tris-HCl
50 mM NaCl
10 mM MgCl2
0.025% Triton® X-100
(pH 7.5 @ 25°C)

Activity in NEBuffers

NEBufferâ„¢ r1.1: 50%
NEBufferâ„¢ r2.1: 100%
NEBufferâ„¢ r3.1: 50%
rCutSmartâ„¢ Buffer: 50%

Diluent Compatibility

Storage Buffer

10 mM Tris-HCl
250 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
0.15% Triton® X-100
pH 7.4 @ 25°C

Heat Inactivation

65°C for 20 minutes

Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive



Companion Products
Materials Sold Separately
Notes
  • Not sensitive to CpG, dcm, or dam methylation.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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