Suggested Loading Protocol for DNA Ladders & Markers

Dilute only 1 µl of DNA Ladder at a time

1. Prepare loading mixture as follows:

   Distilled water (dH20)* or TE Buffer	4 μl
   Gel Loading Dye, Purple (6X), no SDS	1 μl
   DNA Ladder/Marker	1 μl
   Total Volume	6 μl

2. Mix gently by pipetting
3. Load onto the agarose gel

*For multiple loads, dilution, and storage, use TE or other buffer of minimal ionic strength instead of water. DNA may denature if diluted and stored in dH₂0.

This protocol is recommended for a 5mm wide gel lane. The components of the mixture should be scaled up or down, depending on the width of the lane.