We recommend dissolving or diluting target DNA or RNA in H2O and adding 1–5 µl to 25 µl LAMP reactions. TE buffer (10 mM Tris, 1.0 mM EDTA, pH 7.5–8.0) can be used, but targets dissolved in TE should not be added in volumes higher than 1–2 µl (or <10% of final volume). Similarly, any sample stored in alkaline (pH greater than 8.5) or acidic (pH less than 7) solution should make up no more than 10% of the final reaction volume as a higher amount may affect the pH-based detection. If >5 μl is needed for sufficient copy number, use water or a 0.1X solution of sample buffer.