Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets
The WarmStart Colorimetric LAMP 2X Master Mix is an optimized formulation of Bst 2.0 WarmStart® DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of Loop-Mediated Isothermal Amplification (LAMP) and RT-LAMP reactions. This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow (an overview of LAMP and primer design can be found in our video library, https://www.neb.com/tools-and-resources/video-library). This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15–40 minutes.
The following reagents are supplied with this product:
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased product to perform loop-mediated amplification (“LAMP”) and reverse transcription loop-mediated amplification (“RT-LAMP”) for research use only. LAMP is a patented technology belonging to Eiken Chemical Co., Ltd and any other use other than research may require a license from Eiken Chemical Co., Ltd.
Nucleic acid-based aptamers for use with thermophilic DNA polymerases and reverse transcriptase are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA) for Research Use Only (RUO). Commercial use of this product may require a license from New England Biolabs, Inc. For additional information or to inquire about commercial use, please contact busdev@neb.com.