We have validated the columns from the following Monarch kits for use on common benchtop vacuum manifolds: Plasmid Miniprep Kit (NEB #T1010), DNA Gel Extraction Kit (NEB #T1020), PCR & DNA Cleanup Kit (NEB #T1030), and RNA Cleanup Kits (NEB #T2030, #T2040, #T2050). Vacuum manifolds can be utilized with other Monarch columns, but may require optimization and/or additional time for liquid to completely flow through.