New England Biolabs Canada
Expand menu
Sign In or Sign UpMy NEB

FAQ: Which ROX should I use for my qPCR machine?

We provide separate tubes of ROX normalization dye at two concentrations (Low, High) to enable use on all qPCR platforms. Please refer to the table below to determine whether ROX is required, and if so, which concentration should be used:

qPCR Instrument

ROX

Bio-Rad® iQ™5, CFX96, CFX384, Opticon
Roche Lightcycler®
Qiagen Rotor-Gene™
Eppendorf Mastercycler®
Cepheid® SmartCycler®

Not recommended

Applied Biosystems® 7500, QuantStudio™, ViiA7™
Agilent Mx™

Low ROX

Applied Biosystems® 7000, 7300, 7700, 7900HT, StepOne™, StepOnePlus™

High ROX
Top