We recommend running 2 dilutions of each library, and running each dilution in triplicate. Therefore 12 (using 6 standards) or 13 (using 4 standards) libraries can be quantitated in a 96-well plate. Once a workflow is well established, a single dilution could be run, allowing 25 (using 6 standards) or 27 (using 4 standards) libraries per 96-well plate.