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pBR322 Vector

Catalog # Concentration Size List Price Quantity Your Price
N3033L 1000 µg/ml 250 µg $492.00
$442.80
N3033S 1000 µg/ml 50 µg $122.00
$109.80
Catalog # Size List Price Your Price
N3033L 250 µg $492.00
$442.80
N3033S 50 µg $122.00
$109.80
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • View sequence details
  • pBR322 DNA is a commonly used plasmid cloning vector in E. coli (1).
  • The molecule is a double-stranded circle 4,361* base pairs in length (2).
  • pBR322 contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol.
  • The molecular weight is 2.83 x 106 daltons. 

*Sequencing data from Watson (confirmed at New England Biolabs, Inc.) has shown pBR322 to be 4,361 base pairs, not 4,363 base pairs as previously reported.

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  • Monarch_MiniprepProtocol_VideoThumb
    Traditional Cloning Workflow

DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.

Product Source
pBR322 is isolated from E. coli ER2686 (dam +dcm + EcoK M-) by a standard plasmid purification procedure.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  pBR322 Vector N3033SVIAL -20 1 x 0.05 ml 1,000 µg/ml
  pBR322 Vector N3033LVIAL -20 1 x 0.25 ml 1,000 µg/ml

Properties & Usage

Copy Number

Medium (10-100)

Origin of Replication

  • pMB1

Lac Repressor on Plasmid

0

Storage Buffer

10 mM Tris-HCl
1 mM EDTA
pH 8 @ 25°C

Molecular Weights

Apparent: 2.83 daltons
Theoretical: 2.83 daltons


Companion Products
References
  • Bolivar, F., Rodriguez, R.L., Greene, P.J., Betlach, M.C., Heynecker, H.L. and Boyer, H.W. (1977). Gene. 2, 95-113.
  • Watson, N. (1988). Gene. 70, 399-403.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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