WarmStart® LAMP Kit (DNA & RNA)

Catalog #	Concentration	Size	List Price	Quantity	Your Price
E1700L		500 reactions	$1,398.00		$1,258.20
E1700S		100 reactions	$349.00		$314.10

Catalog #	Size	List Price	Your Price
E1700L	500 reactions	$1,398.00	$1,258.20
E1700S	100 reactions	$349.00	$314.10

The WarmStart LAMP Kit (DNA & RNA) is designed to provide a simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provides rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase. This kit is supplied with the WarmStart LAMP 2X Master Mix, which contains a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized LAMP buffer solution. Both Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase have been engineered for improved performance in LAMP and RT-LAMP reactions. A fluorescent dye is also supplied to enable real-time fluorescence measurement of LAMP. The WarmStart LAMP Kit is compatible with multiple detection methods, including turbidity detection, real-time fluorescence detection (when used with LAMP fluorescent dye) and end-point visualization.

This product is also available in a lyophilized format. The LyoPrime WarmStart® Fluorescent LAMP/RT-LAMP Mix (with UDG) is a lyophilized LAMP mix offering ambient/room temperature storage and shipping and streamlined assay development. To learn about the lyophilization process, please visit www.neb.com/lyoprime.

The WarmStart LAMP Kit (DNA & RNA) includes fluorescent dye for real-time fluorescence measurement. Alternately, detection can be accomplished by turbidity detection or a variety of endpoint visualization methods.

For carryover contamination prevention, a version containing dUTP and thermolabile UDG is available: WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG) (NEB #E1708).

speed and sensitivity — A RNA target (HMBS2) was amplified from Jurkat total RNA using the WarmStart LAMP Kit and OptiGene Master Mix (ISO-001). Reactions were performed at 65°C for 74 minutes on a real-time thermocycler (Bio-Rad CFX96) in triplicate. Time to result is set as the time at which the fluorescence crossed a threshold of 10% of maximal fluorescence. NEB’s WarmStart LAMP Kit resulted in faster and more robust detection as compared to the OptiGene Master Mix.

Fast LAMP/RT-LAMP results — Experiments were designed to amplify a DNA target (BRCAb) and a RNA target (HMBS2) by LAMP or RT-LAMP, respectively. A 25 μl reaction containing 10 ng HeLa DNA or 5 ng Jurkat total RNA, 1X Master Mix, 1X LAMP primers and fluorescent dye was incubated on a Bio-Rad CFX98 instrument at 65°C for 75 minutes. Lucigen® OmniAmp® RNA & DNA Kit and OptiGene Isothermal Master Mix (ISO-001 ) were run according to manufacturers’ recommendations.

The WarmStart LAMP Kit (DNA & RNA) enables specific amplification across multiple species/targets

PRIMER SET	DNA TEMPLATE
PRIMER SET	HeLa DNA	Lyme DNA	B. malayi DNA	E. coli DNA	C. elegans DNA
BRCAb (human)	+
CFTR (human)	+
LymO (Lyme)		+
BmHhal (B. malayi)			+
dnaE (E. coli)				+
Lec10b (C. elegans)					+
Lec6 (C. elegans)					+

In each individual reaction, multiple gDNA templates were added along with primers specific to a target in only one species. Reactions were incubated at 65°C for 74 minutes on a thermocycler (Bio-Rad CFX96) in triplicate. Positive results (+) were observed only in the expected species/target combinations, demonstrating high specificity of the WarmStart LAMP reactions.

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

E1700S -20

	WarmStart LAMP 2X Master Mix	M1700SVIAL	-20	1 x 1.25 ml	2 X
	LAMP Fluorescent Dye	B1700AVIAL	-20	1 x 0.05 ml	50 X

E1700L -20

	WarmStart LAMP 2X Master Mix	M1700SVIAL	-20	5 x 1.25 ml	2 X
	LAMP Fluorescent Dye	B1700SVIAL	-20	1 x 0.25 ml	50 X

Properties & Usage

Materials Required but not Supplied

LAMP Primers (we recommend using NEB LAMP Primer Design Tool)
Target Nucleic Acid Samples
Molecular Biology Grade H₂O
Heat block, water bath, real-time turbidimeter or thermocycler (with real-time fluorescence measurement if desired) and instrument-appropriate reaction vessels

Companion Products

References

Notomi, T., et al. (2000). Nucleic Acids Res. 28, PubMedID: E63
Nagamine, K., et al. (2001). Clin. Chem. 47, 1742-1743.
Nagamine, K., Hase, T., Notomi, T. (2002). Mol. Cell Probes. 16, 223-229.
Mori, Y., Nagamine, K., Tomita, N., Notomi, T. (2001). Biochem. Biophys. Res. Commun. 289, 150-154.
Mori, Y., Kitao, M., Tomita, N., Notomi, T. (2004). J. Biochem. Biophys. Methods. 59, 145-157.
Tomita, N., Mori, Y., Kanda, H., Notomi, T. (2008). Nat. Protoc. 3, 877-882.
Tanner, N.A. and Evans, T. C. Jr (2014). Curr. Protoc. Mol. Biol. 105, Unit 15.14.
Hsieh et al. (2014). Chem. Comm. 50, 3747-3749.
Tanner, N.A., Zhang, Y. and Evans, T.C. Jr (2015). Biotechniques. 58, 59-68.

Protocols

WarmStart LAMP Kit (DNA & RNA) Protocol (E1700)

DataCards

manualE1700

FAQs

Citations

See more details on Bioz

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Change Notifications

The M1700 component is now supplied in Sarstedt vials (February 28, 2022).

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

E1700S_L_v3

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased product to perform loop-mediated amplification (“LAMP”) and reverse transcription loop-mediated amplification (“RT-LAMP”) for research use only. LAMP is a patented technology belonging to Eiken Chemical Co., Ltd and any other use other than research may require a license from Eiken Chemical Co., Ltd.

Nucleic acid-based aptamers for use with thermophilic DNA polymerases and reverse transcriptases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the WarmStart^® LAMP Kit (DNA & RNA) for Research Use Only (RUO). Commercial use of this product may require a license from New England Biolabs, Inc. For additional information or to inquire about commercial use, please contact busdev@neb.com.