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T7 RNA Polymerase (High Concentration)

Catalog # Concentration Size List Price Quantity Your Price
M0460T 1000000 units/ml 50000 units $885.00
$796.50
Catalog # Size List Price Your Price
M0460T 50000 units $885.00
$796.50
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

In vitro transcription using T7 phage promoter 

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T7 RNA Polymerase (High Concentration) is offered at a 20-fold higher concentration than our standard T7 RNA Polymerase (NEB #M0251) and is ideal for experienced users interested in building and optimizing their own in vitro transcription reactions. The enzyme is accompanied by RNA Polymerase Reaction Buffer and magnesium chloride solution to further enable testing of different conditions such as nucleotide concentrations. 

For standard RNA synthesis and high yield reactions, we recommend our T7 RNA Polymerase (NEB #M0251) and HiScribe®; kits. The HiScribe kits are specifically designed to produce high quality, high yield RNA in a short time with minimal optimization. 

In vitro transcription involves multiple components in addition to T7 RNA Polymerase including ribonucleotides, inorganic pyrophosphatase and RNase Inhibitor, which can be purchased separately. Please refer to the Related Products section for details. More information regarding transcription optimization can be obtained from the following article.  

Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the T7 phage promoters. The 99 kD enzyme catalyzes in vitro RNA synthesis from a cloned DNA sequence under the T7 promoters. RNA produced using the T7 RNA Polymerase is suitable for many applications in research and biotechnology. 

Product Source
Isolated from E.coli carrying a plasmid which contains the T7 RNA Polymerase gene. 
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  T7 RNA Polymerase (High Concentration) M0460TVIAL -20 1 x 0.05 ml 1,000,000 units/ml
  RNAPol Reaction Buffer B9012SVIAL -20 1 x 1 ml 10 X
  MgCl2 Solution B2534AVIAL -20 1 x 0.5 ml 0.2 M
Application Features
  • mRNA for in vitro translation and micro injection 
  • Radiolabeled RNA probes
  • Non-isotopic RNA labeling
  • Preparation of RNA vaccines
  • Guide RNA for gene targeting
  • RNA structure, processing and catalysis studies
  • Anti-sense RNA for gene expression experiment 
  • RNA amplification
  • Isothermal amplification
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Companion Products
References
  • Yin, Y and Carter, C.W. (1996). Nucl. Acids Res. 24, 7, 1279–1286.
  • Schenborn, E.T. and Meirendorf, R.C. (1985). Nucl. Acids Res. 13, 6223-6236.
  • Davanloo, P., et al. (1984). Proc. Natl. Acad. Sci. USA. 81, 2035-2039.
  • Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. 2nd Ed, 10.27-10.37.
  • Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989).  Molecular Cloning: A Laboratory Manual. 2nd Ed, 18.82-18.84.
  • Melton, D.A., et al. (1984). Nucl. Acids Res. 12, 7035-7056.
  • Milligan, J.F., et al. (1987). Nucl. Acids Res. 15, 8783.
  • Noren, C.J. et al. (1990). Nucl. Acids Res. 18, 83-88.
  • Kreig, P.A. and Melton, D.A. (1984). Nucl. Acids Res. 12, 7057-7070.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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