T3 DNA Ligase will ligate these substrates:
dsDNA
(1)Nicked DNA/RNA
1. Note: Trace amounts of this substrate can be ligated with overnight incubation, and ligation is further enhanced in Stick Together DNA Ligase Buffer. Please note that Blunt TA Ligase Mastermix is the recommended ligase product for this substrate.
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
1X StickTogether™ DNA Ligase Buffer
Incubate at 25°C
1X StickTogether™ DNA Ligase Buffer
66 mM Tris-HCl
10 mM MgCl2
1 mM ATP
1 mM DTT
7.5% Polyethylene glycol (PEG 6000)
(pH 7.6 @ 25°C)
10 mM Tris-HCl
50 mM KCl
1 mM DTT
50% Glycerol
0.1 mM EDTA
pH 7.4 @ 25°C
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