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Endonuclease VIII

Catalog # Concentration Size List Price Your Price
M0299L 10000 units/ml 5000 units $527.00
$474.30
M0299S 10000 units/ml 1000 units $128.00
$115.20
Catalog # Size List Price Your Price
M0299L 5000 units $527.00
$474.30
M0299S 1000 units $128.00
$115.20
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

  • Bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities
  • The N-glycosylase activity releases damaged pyrimidines, including thymine glycol and uracil glycol. The AP lyase activity cleaves DNA phosphodiester backbone at AP sites via β and δ-elimination, creating a 1 nucleotide DNA gap with 5' and 3' phosphate termini
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    What are endonucleases and their applications?
Endonuclease VIII from E. coli acts as both an N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged pyrimidines from double-stranded DNA, generating an apurinic (AP site). The AP-lyase activity cleaves 3´ and 5´ to the AP site leaving a 5´ phosphate and a 3´ phosphate. Damaged bases recognized and removed by Endonuclease VIII include urea, 5, 6- dihydroxythymine, thymine glycol, 5-hydroxy-5- methylhydantoin, uracil glycol, 6-hydroxy-5, 6-dihydrothymine and methyltartronylurea (1,2). While Endonuclease VIII is similar to Endonuclease III, Endonuclease VIII has β and δ lyase activity while Endonuclease III has only β lyase activity.
Product Source
An E. coli strain which carries the cloned nei gene.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Endonuclease VIII M0299SVIAL -20 1 x 0.1 ml 10,000 units/ml
  Endonuclease VIII Reaction Buffer B0299SVIAL -20 1 x 1.5 ml 10 X
  Endonuclease VIII M0299LVIAL -20 1 x 0.5 ml 10,000 units/ml
  Endonuclease VIII Reaction Buffer B0299SVIAL -20 1 x 1.5 ml 10 X
Application Features
  • Single cell gel electrophoresis (Comet assay) (3,4,5)
  • Alkaline elution (6)
  • Alkaline unwinding (7)


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Notes
  • Recommended Dilution for Comet Assay: 1:104 to 1:105 (3,4,5,8). For a protocol please visit: http://cometassay.com
  • Endonuclease VIII has full activity in CutSmart Buffer (NEB #B7204). Endo VIII has a 75% activity in NEBuffer 1 (NEB #B7001), 75% activity in NEBuffer 4 (NEB #B7004), 50% activity in NEBuffer 2 (NEB #B7002). The use of Endonuclease VIII in NEBuffer 3 (NEB #B7003) is not recommended. 
References
  • Dizdaroglu, M., Laval, J. and Boiteux, S. (1993). Biochemistry . 32, 12105-12111.
  • Hatahet, Z. et al. (1994). J. Biol. Chem.. 269, 18814-18820.
  • Singh, N. et al. (1988). Exp. Cell. Res. . 175, 184-191.
  • Collins, A. et al. (1993). Carcinogenesis . 14, 1733-1735.
  • Collins, A. et al. (1996). Environ. Health Perspect . 104, 465-469.
  • Pflaum, M. et al. (1998). Free Rad. Res. . 29, 585-594.
  • Hartwig, A. et al. (1996). Toxicol. Lett.. 88, 85-90.
  • Marks, K. unpublished observation.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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