New England Biolabs Canada
Expand menu
Sign In or Sign UpMy NEB

We are transitioning to our new website at www.neb.com, which will go live on April 7th, 2025.

On April 2nd at 8:00 PM ET, www.neb.ca e-commerce functionality will be disabled for the website transition. If you need to place your order between April 2nd at 8:00 pm ET to April 6th, then please call 1-800-387-1095 or email orders.ca@neb.com.

The transition to the new website will not affect our shipping schedule. If you're a registered user, all your account details, including order history and shipping details will be safely transferred to the new site.

T4 RNA Ligase 1 (ssRNA Ligase)

Catalog # Concentration Size List Price Quantity Your Price
M0204L 10000 units/ml 5000 units $456.00
$410.40
M0204S 10000 units/ml 1000 units $114.00
$102.60
Catalog # Size List Price Your Price
M0204L 5000 units $456.00
$410.40
M0204S 1000 units $114.00
$102.60
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
T4 RNA Ligase 1 catalyzes the ligation of a 5´ phosphoryl-terminated nucleic acid donor to a 3´ hydroxyl-terminated nucleic acid acceptor through the formation of a 3´ → 5´ phosphodiester bond with hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA and DNA as well as dinucleoside pyrophosphates.
Featured Videos
View Video Library
  • Monarch_MiniprepProtocol_VideoThumb
    Avoiding RNase Contamination
 
Product Source
An E. Coli strain that carries the T4 RNA Ligase I gene
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  T4 RNA Ligase 1 (ssRNA Ligase) M0204SVIAL -20 1 x 0.1 ml 10,000 units/ml
  T4 RNA Ligase Reaction Buffer B0216SVIAL -20 1 x 1.5 ml 10 X
  Adenosine-5'-Triphosphate (ATP) B0756AVIAL -20 1 x 0.2 ml 10 mM
  50% PEG 8000 B1004SVIAL -20 1 x 1 ml 1 X
  T4 RNA Ligase 1 (ssRNA Ligase) M0204LVIAL -20 1 x 0.5 ml 10,000 units/ml
  T4 RNA Ligase Reaction Buffer B0216SVIAL -20 1 x 1.5 ml 10 X
  50% PEG 8000 B1004SVIAL -20 2 x 1 ml 1 X
  Adenosine 5'-Triphosphate (ATP) P0756SVIAL -20 1 x 1 ml 10 mM
Application Features
  • Labeling of 3'-termini of RNA with 5'-[32P] pCp (3)
  • Inter- and intramolecular joining of RNA and DNA molecules (4,5)
  • Synthesis of single-stranded oligodeoxyribo-nucleotides (6)
  • Incorporation of unnatural amino acids into proteins (7)
  • Ligation of ss-RNA and DNA


Companion Products
Materials Sold Separately
Notes
  • Addition of DMSO to 10% (v/v) is required for pCp ligation (3).
References
  • England, T., Gumport, R. and Uhlenbeck, O. (1977). Proc. Natl. Acad. Sci. USA. 74, 4839-4842.
  • Rand, K.N. and Gait, M.J. (1984). EMBO J.. 3, 397-402.
  • England, T. and Uhlenbeck, O. (1978). Nature. 275, 560-562.
  • Romaniuk, P. and Uhlenbeck, O. (1983). R. Wu, L. Grossman and K. Moldave(Ed.), Methods Enzymol.. 100, 52-56. New York: Academic Press.
  • Moore, M.J. and Sharp, P.A. (1992). Science. 256, 992-997.
  • Tessier, D.C., Brousseau, R., and Vernet, T. (1986). Anal. Biochem.. 158, 171-178.
  • Noren, C.J. et al. (1989). Science. 244, 182-188.
  • Silber, R., Malathi, B.G. and Hurwitz, J. (1972). Proc. Natl. Acad. Sci. USA. 69, 3009-3013.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Change Notifications
Effective October 28, 2024, activity assay changed.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Other Products You May Be Interested In
 Top