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NEBNext® Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs)

Catalog # Concentration Size List Price Quantity Your Price
E7140L 96 reactions $844.00
$759.60
E7140S 24 reactions $212.00
$190.80
Catalog # Size List Price Your Price
E7140L 96 reactions $844.00
$759.60
E7140S 24 reactions $212.00
$190.80
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

NEBNext Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs) is compatible with Illumina® sequencing and includes the NEBNext EM-seq™ Adaptor, and NEBNext PCR primers that enable incorporation of unique index pairs during library amplification.  The index primers are provided as pre-mixed unique pairs of i5 and i7 index primers, packaged in a single-use 96-well plate with a pierceable foil seal, or in individual vials.

Although optimized for the EM-seq workflow, the NEBNext EM-seq Adaptor is also compatible with bisulfite sequencing workflows.

These components are also included in the NEBNext Enzymatic Methyl-seq Kit (NEB #E7120).

  • Optimized for the EM-seq workflow
  • Compatible with bisulfite sequencing workflows
  • Addresses the “barcode hopping” issue seen with some Illumina sequencing instruments
  • Increases sample identification specificity with unique dual index primer pairs
NEBNext Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs) is compatible with Illumina® sequencing and includes the NEBNext EM-seq™ Adaptor, and NEBNext PCR primers that enable incorporation of unique index pairs during library amplification.

The 96-reaction size includes a single-use 96-well plate with a pierceable foil seal. Each well of the plate contains a unique pair of one i5 index primer and one i7 index primer, therefore providing 96 unique dual indices. For the 24-reaction size, indices are provided in 24 separate vials, with each vial containing a unique pair of one i5 index primer and one i7 index primer. There is no overlap between i5 and i7 index pairs provided in the 24 and 96 reaction kits. 

Although optimized for the EM-seq workflow, the NEBNext EM-seq Adaptor is also compatible with bisulfite sequencing workflows.


These components are also provided in the NEBNext Enzymatic Methyl-seq Kit (NEB #E7120).
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  EM-seq™ Index Primer 1 (S) E7141AVIAL -20 1 x 0.005 ml 10 µM
  EM-seq™ Index Primer 2 (S) E7142AVIAL -20 1 x 0.005 ml 10 µM
  EM-seq™ Index Primer 3 (S) E7143AVIAL -20 1 x 0.005 ml 10 µM
  EM-seq™ Index Primer 4 (S) E7144AVIAL -20 1 x 0.005 ml 10 µM
  EM-seq™ Index Primer 5 (S) E7145AVIAL -20 1 x 0.005 ml 10 µM
  EM-seq™ Index Primer 6 (S) E7146AVIAL -20 1 x 0.005 ml 10 µM
  EM-seq™ Index Primer 7 (S) E7147AVIAL -20 1 x 0.005 ml 10 µM
  EM-seq™ Index Primer 8 (S) E7148AVIAL -20 1 x 0.005 ml 10 µM
  EM-seq™ Index Primer 9 (S) E7149AVIAL -20 1 x 0.005 ml 10 µM
  EM-seq™ Index Primer 10 (S) E7150AVIAL -20 1 x 0.005 ml 10 µM
  EM-seq™ Index Primer 11 (S) E7151AVIAL -20 1 x 0.005 ml 10 µM
  EM-seq™ Index Primer 12 (S) E7152AVIAL -20 1 x 0.005 ml 10 µM
  EM-seq™ Index Primer 13 (S) E7153AVIAL -20 1 x 0.005 ml 10 µM
  EM-seq™ Index Primer 14 (S) E7154AVIAL -20 1 x 0.005 ml 10 µM
  EM-seq™ Index Primer 15 (S) E7155AVIAL -20 1 x 0.005 ml 10 µM
  EM-seq™ Index Primer 16 (S) E7156AVIAL -20 1 x 0.005 ml 10 µM
  EM-seq™ Index Primer 17 (S) E7157AVIAL -20 1 x 0.005 ml 10 µM
  EM-seq™ Index Primer 18 (S) E7158AVIAL -20 1 x 0.005 ml 10 µM
  EM-seq™ Index Primer 19 (S) E7159AVIAL -20 1 x 0.005 ml 10 µM
  EM-seq™ Index Primer 20 (S) E7160AVIAL -20 1 x 0.005 ml 10 µM
  EM-seq™ Index Primer 21 (S) E7161AVIAL -20 1 x 0.005 ml 10 µM
  EM-seq™ Index Primer 22 (S) E7162AVIAL -20 1 x 0.005 ml 10 µM
  EM-seq™ Index Primer 23 (S) E7163AVIAL -20 1 x 0.005 ml 10 µM
  EM-seq™ Index Primer 24 (S) E7164AVIAL -20 1 x 0.005 ml 10 µM
  NEBNext® EM-seq™ Adaptor E7165AVIAL -20 1 x 0.06 ml
  NEBNext® 96 Unique Dual Index Primer Pairs Plate (L) E7166AVIAL -20 1 x 5 μl/well 10 µM
  NEBNext® EM-seq™ Adaptor E7165AAVIAL -20 1 x 0.24 ml
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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