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Tte UvrD Helicase

Catalog # Concentration Size List Price Quantity Your Price
M1202S 20 µg/ml 0.50 µg $117.00
$105.30
Catalog # Size List Price Your Price
M1202S 0.50 µg $117.00
$105.30
Catalog #
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca


  • Unwinds double-stranded DNA
  • Thermostable to 65°C
  • Reduces non-specific product formation in isothermal amplification (e.g. LAMP)
This is an Enzyme for Innovation (EFI). EFI is a project initiated by NEB to provide unique enzymes to the scientific community in the hopes of enabling the discovery of new and innovative applications. These enzymes have interesting properties and unique specificities.
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Tte UvrD Helicase is a repair helicase capable of unwinding double-stranded DNA, without a requirement for a specific flap or overhang structure, from the thermophilic organism Thermoanaerobacter tengcongensis. It is active on a wide range of DNA substrates and, along with its thermostability (active to 70°C), Tte UvrD Helicase has been demonstrated to be a useful additive for improving specificity of isothermal amplification reactions, particularly in conjunction with the WarmStart® LAMP Kit (DNA & RNA).

Tte UvrD Helicase Eliminates Non-Template Amplification in LAMP Reactions



LAMP reactions were performed using the WarmStart® LAMP Kit (DNA & RNA) (NEB #E1700) and a primer set prone to high levels of non-template amplification. Without Tte UvrD Helicase (left), the positive reaction (+DNA) amplifies in ~8 minutes while the no-template control (NTC) amplifies in ~20 minutes. In the presence of 10 ng Tte UvrD Helicase (right), the positive reaction maintains its rapid amplification time with a slight reduction in total RFU, while the NTC reaction is completely suppressed.


Product Source
Tte UvrD Helicase is isolated from a strain of E. coli which carries the cloned UvrD gene from Thermoanaerobacter tengcongensis.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Tte UvrD Helicase M1202SVIAL -20 1 x 0.025 ml
  Isothermal Amplification Buffer Pack B0537SVIAL -20 1 x 1.5 ml 10 X
  Adenosine 5'-Triphosphate (ATP) P0756SVIAL -20 1 x 1 ml 10 mM
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

References
  • An, L., Tang, W., Ranalli, T.A., Kim, H.J., Wytiaz, J., and Kong, H. (2005). Characterization of a thermostable UvrD helicase and its participation in helicase-dependent amplification. J Biol Chem. 280 (32), 28952-8. PubMedID: 15955821
  • Mauris, J., and Evans, T.C. Jr. (2010). A human PMS2 homologue from Aquifex aeolicus stimulates an ATP-dependent DNA helicase. J Biol Chem. 285 (15), 11087-92. PubMedID: 20129926
  • Vincent, M., Xu, Y., and Kong, H. (2004). Helicase-dependent isothermal DNA amplification. EMBO Rep. 5 (8), 795-800. PubMedID: 15247927
Citations
Additional Citations
  • Zaczek-Moczydłowska, M.A.; Mohamed-Smith, L.; Toldrà, A.; Hooper, C.; Campàs, M.; Furones, M.D.; Bean, T.P.; Campbell, K. (2020) A Single-Tube HNB-Based Loop-Mediated Isothermal Amplification for the Robust Detection of the Ostreid herpesvirus 1. Int J Mol Sci 21, 6605,
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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