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APE 1

Catalog # Concentration Size List Price Your Price
M0282L 10000 units/ml 5000 units $505.00
$454.50
M0282S 10000 units/ml 1000 units $124.00
$111.60
Catalog # Size List Price Your Price
M0282L 5000 units $505.00
$454.50
M0282S 1000 units $124.00
$111.60
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

  • Apurinic/apyrimidinic Endonuclease 1
  • DNA AP endonuclease that catalyzes the cleavage of DNA phosphodiester backbone at AP sites via hydrolysis leaving a 1 nucleotide gap with 3'-hydroxyl and 5' deoxyribose phosphate (dRP) termini
  • APE 1 has also been reported to have DNA 3'-diesterase, 3' to 5' exonuclease, and RNase H activities
  • Also known as HAP1 or Ref-1
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  • Monarch_MiniprepProtocol_VideoThumb
    What are endonucleases and their applications?
Human apurinic/apyrimidinic (AP) endonuclease, APE 1, also known as HAP 1 or Ref-1, shares homology with Escherichia coli exonuclease III protein. APE 1 cleaves the phosphodiester backbone immediately 5´ to an AP site, via hydrolytic mechanism, to generate a single-strand DNA break leaving a 3´-hydroxyl and 5´-deoxyribose phosphate terminus. Besides AP endonuclease activity, APE 1 has also been reported to have weak DNA 3´ -diesterase, 3´ to 5´ exonuclease and RNase H activities (3-5).

In addition to DNA repair activity, APE 1 is also capable of regulating the DNA binding activity of many transcription factors in vitro by a redox mechanism (Ref-1). As part of this process, APE 1 has been shown to stimulate the DNA binding activity of Fos-Jun heterodimers, Jun-Jun homodimers and Hela cell AP-1 proteins as well as that of several other transcription factors including NF-kB, Myb and members of the ATF/CREB family (7-9).
Product Source
An E. coli strain which carries the cloned human APE 1 gene.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  APE 1 M0282SVIAL -20 1 x 0.1 ml 10,000 units/ml
  NEBuffer™ 4 B7004SVIAL -20 1 x 1.25 ml 10 X
  APE 1 M0282LVIAL -20 1 x 0.5 ml 10,000 units/ml
  NEBuffer™ 4 B7004SVIAL -20 1 x 1.25 ml 10 X
Application Features
  • Single cell gel electrophoresis (Comet assay)
  • Alkaline elution
  • Alkaline unwinding
  • Modified nick translation


Companion Products
Materials Sold Separately
Notes
  • Recommended Dilution for the Comet Assay: 1:103. For a protocol please visit: http://cometassay.com
  • The enzyme has 50% activity in NEBuffers 1-3. (NEB #B7001, NEB #B7002, NEB #B7003)
References
  • Robson, C.N. and Hickson, D.I. (1991). Nucl. Acids Res.. 19, 5519-5523.
  • Flaherty, D.M. (2001). Am. J. Respir. Cell. Mol. Biol.. 25, 664-667.
  • Demple, B. et al. (1991). Proc. Natl. Acad. Sci. USA. 88, 11450-11454.
  • Barzilay, G. et al. (1995). Nucl.Acids Res.. 23, 1544-1550.
  • Barzilay, G. et al. (1995). NatureStruc. Biol.. 2, 451-468.
  • Gorman, M.A. et al (1997). EMBOJ.. 16, 6548-6558.
  • Xanthoudakis, S. et al. (1992). EMBO J.. 11, 3323-3335.
  • Walker, L.J. et al. (1993). Mol.Cell. Biol.. 13, 5370-5376.
  • Vidal, A.E. (2001). EMBO J.. 20, 6530-6539.
  • Wilson, D.M. III et al. (1995). J. Biol. Chem.. 270, 16002-16007.
Usage Guidelines
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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