Mung Bean Nuclease

Product Information

• Reagents Supplied

  The following reagents are supplied with this product:

  NEB https://www.neb.com/en/#	Component Name	Component https://www.neb.com/en/#	Stored at (°C)	Amount	Concentration
  M0250S     -20       Mung Bean Nuclease M0250SVIAL -20 1 x 0.15 ml 10,000 units/ml   Mung Bean Nuclease Reaction Buffer B0250SVIAL -20 1 x 1 ml 10 X
  M0250L     -20       Mung Bean Nuclease M0250LVIAL -20 1 x 0.75 ml 10,000 units/ml   Mung Bean Nuclease Reaction Buffer B0250SVIAL -20 1 x 1 ml 10 X

• Properties & Usage

  Unit Definition

  One unit is defined as the amount of enzyme required to digest 1 µg of M13mp18 single-stranded DNA to fragments less than 1 kb in length in a total reaction volume of 80 µl in 1X Mung Bean Nuclease Reaction Buffer when incubated for 15 minutes at 37°C.

  Reaction Conditions

  1X Mung Bean Nuclease Reaction Buffer
  Incubate at 30°C

  1X Mung Bean Nuclease Reaction Buffer
  30 mM NaCl
  50 mM sodium acetate
  1 mM ZnSO₄
  (pH 5 @ 25°C)

  Storage Buffer

  10 mM sodium acetate
  0.1 mM Zinc Acetate
  1 mM Cysteine
  50% Glycerol
  0.001% Triton® X-100
  pH 5 @ 25°C

  Molecular Weight

  Theoretical: 39 kDa
  

• Advantages and Features

  Application Features

  • Removal of 3´ and 5´ extensions or single-stranded regions from DNA or RNA termini
  • Transcriptional mapping
  • Cleavage of hairpin loops
  • Excision of gene coding sequences from genomic DNA
  • Generation of new restriction sites

• Related Products

  Materials Sold Separately

  • Mung Bean Nuclease Reaction Buffer

• Product Notes

  1. It is no longer necessary to supplement Mung Bean Nuclease reactions with Zn²⁺. The zinc acetate in the storage buffer fulfills the Zn²⁺ requirement of the enzyme even after dilution in a reaction.
  2. Do not attempt to heat inactivate, DNA will “breathe” before enzyme inactivates, causing undesirable degradation. 

• References

  1. Kowalski, D. et al. (1976). Biochemistry. 15, 4457-4463.
  2. McCutchan, T.F. et al. (1984). Science. 225, 626-628.

Protocols, Manuals & Usage

• Protocols

  1. Removal of Single-Stranded Extension Protocol using Mung Bean Nuclease (M0250)

• Usage Guidelines

  • Traditional Cloning Quick Guide

Tools & Resources

• Selection Charts

  • Activities of Exonucleases and Non-specific Endonucleases
  • Blunting Selection Chart
  • Common Applications for Exonucleases and Endonucleases
  • Properties of Exonucleases and Non-specific Endonucleases

• Web Tools

  • Exo Selector
  • NEBcloner^®

FAQs & Troubleshooting

• FAQs

  1. Should I use Mung Bean Nuclease or DNA Polymerase I, Large (Klenow) Fragment (NEBhttps://www.neb.com/en/# M0210)?
  2. Is the Mung Bean Nuclease active in other NEBuffers?
  3. Can Mung Bean Nuclease be heat inactivated?
  4. Does Zn²⁺ need to be added to a Mung Bean Nuclease reaction?
  5. Will Mung Bean Nuclease degrade double stranded DNA?

Citations & Technical Literature

• Citations

  Product Citation Tool

• Feature Articles

  • The effect of nucleic acid modifications on digestion by DNA exonucleases

Quality, Safety & Legal

• Quality Control Assays

  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

• Specifications & Change Notifications

  Effective May 2, 2022, updated unit definition.

  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

  • M0250S_L_v1
  • M0250S_L_v2

• Certificate of Analysis

  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

  • M0250S_v1_10018062
  • M0250L_v1_10022243
  • M0250S_v1_10035014
  • M0250L_v1_10035015
  • M0250S_v1_10035019
  • M0250L_v1_10044911
  • M0250S_v1_10054611
  • M0250L_v1_10054610
  • M0250S_v1_10070946
  • M0250S_v1_10089432
  • M0250S_v1_10103409
  • M0250S_v1_10106726
  • M0250L_v1_10089435
  • M0250L_v1_10130304
  • M0250S_v1_10130305
  • M0250S_v1_10143533
  • M0250S_v2_10149098
  • M0250L_v2_10157968
  • M0250S_v2_10201096
  • M0250L_v2_10206267
  • M0250S_L_v1_0251712
  • M0250S_v2_10236980
  • M0250S_v2_10263233
  • M0250L_v2_10265458
  • M0250L_v2_10239808

• Safety Data Sheets

  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

  • Mung Bean Nuclease
  • Mung Bean Nuclease Reaction Buffer

• Legal and Disclaimers

  Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.