New England Biolabs Canada
Expand menu
Sign In or Sign UpMy NEB
1

FatI

Catalog # Concentration Size List Price Quantity Your Price
R0650L 2000 units/ml 250 units $626.00
$563.40
R0650S 2000 units/ml 50 units $151.00
$135.90
Catalog # Size List Price Your Price
R0650L 250 units $626.00
$563.40
R0650S 50 units $151.00
$135.90
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • Restriction Enzyme Cut Site: /CATG
Featured Videos
View Video Library
  • Monarch_MiniprepProtocol_VideoThumb
    Reduce Star Activity with High-Fidelity Restriction Enzymes
  • Monarch_MiniprepProtocol_VideoThumb
    Standard Protocol for Restriction Enzyme Digests
  • Monarch_MiniprepProtocol_VideoThumb
    NEB® TV Ep. 15 – Applications of Restriction Enzymes
  • Monarch_MiniprepProtocol_VideoThumb
    Restriction Enzyme Digest Protocol: Cutting Close to DNA End
  • Monarch_MiniprepProtocol_VideoThumb
    Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel
  • Monarch_MiniprepProtocol_VideoThumb
    Why is My Restriction Enzyme Not Cutting DNA?
  • Monarch_MiniprepProtocol_VideoThumb
    Restriction Enzyme Digest Problem: Too Many DNA Bands
  • Monarch_MiniprepProtocol_VideoThumb
    Double Digestion with NEBcloner
Product Source
An E. coli strain that carries the cloned FatI gene from Flavobacterium aquatile NL3 (S.K. Degtyarev)
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  FatI R0650SVIAL -20 1 x 0.025 ml 2,000 units/ml
  NEBuffer™ r2.1 B6002SVIAL -20 1 x 1.25 ml 10 X
  FatI R0650LVIAL -20 1 x 0.125 ml 2,000 units/ml
  NEBuffer™ r2.1 B6002SVIAL -20 1 x 1.25 ml 10 X

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 µg of pUC19 DNA in 1 hour at 55°C in a total reaction volume of 50 µl.

Reaction Conditions

1X NEBuffer™ r2.1
Incubate at 55°C

1X NEBuffer™ r2.1
50 mM NaCl
10 mM Tris-HCl
10 mM MgCl2
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Activity in NEBuffers

NEBufferâ„¢ r1.1: 10%
NEBufferâ„¢ r2.1: 100%
NEBufferâ„¢ r3.1: 50%
rCutSmartâ„¢ Buffer: 50%

Diluent Compatibility

Storage Buffer

10 mM Tris-HCl
50 mM NaCl
0.1 mM EDTA
1 mM DTT
50% Glycerol
200 µg/ml Recombinant Albumin
pH 7.4 @ 25°C

Heat Inactivation

80°C for 20 minutes

Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive

Activity at Temperature

@37°C: 100%


Companion Products
Notes
  • FatI is a neoschizomer of NlaIII.
  • Not sensitive to CpG, dcm, or dam methylation.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Change Notifications
FatI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10201943. All subsequent (higher number) lots will contain rAlbumin. For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin).
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Other Products You May Be Interested In
 Top