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Boletopsis grisea Lectin (BGL)

Catalog # Concentration Size List Price Quantity Your Price
P0867S 1 mg/ml 1 ml $328.00
$295.20
Catalog # Size List Price Your Price
P0867S 1 ml $328.00
$295.20
Catalog #
Qty:
 
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  • Binds to N-glycans having at least one terminal outer arm GlcNAc as well as O-glycans containing Gal-β1,3-GalNAc-α-  within their structure.
  • Enables enrichment of GlcNAc-capped N-glycans or mucin type O-glycopeptides from complex samples in glycomics and glycoproteomics analytical workflows. 
  • This is an Enzyme for Innovation (EFI). EFI is a project initiated by NEB to provide unique enzymes to the scientific community in the hopes of enabling the discovery of new and innovative applications. These enzymes have interesting properties and unique specificities.
 
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Boletopsis grisea Lectin (BGL) is a recombinant 15 kDa lectin from the Boletopsis grisea mushroom that has been expressed in E. coli.  BGL has two separately functioning ligand binding sites (1).  Site 1 binds to O-glycans bearing the Tn antigen (GalNAc-α-Ser/Thr) or Thomsen-Friedenreich antigen (TF-antigen; Gal-β1,3-GalNAc-α-) and Site 2 binds N-glycans with terminal GlcNAc residues.

Boletopsis grisea Lectin (BGL) binding sites



BGL has two separately functioning ligand binding sites (1). Site 1 binds to O-glycans bearing the Tn antigen (GalNAc-α-Ser/Thr) or Thomsen-Friedenreich antigen (TF-antigen; Gal-β1,3-GalNAc-α-) and Site 2 binds N-glycans with terminal GlcNAc residues.


Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Boletopsis grisea Lectin (BGL) P0867SVIAL -20 1 x 1 ml 1 mg/ml
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

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Notes
  • BGL has only been tested for the enrichment of O-glycopeptides and free N-glycans in EDGE assays.
  • Optimal incubation time (60 to 90 minutes) will depend on the specific substrate.
  • A lower starting amount of BGL for the EDGE assay could be used for O-glycopeptides. This amount should be empirically determined.
  • The formulation of BGL contains 50 mM Tris-HCl, pH 7.5 and 200 mM NaCl. If this salt concentration will affect downstream workflow (e.g., capillary electrophoresis analysis), buffer exchange treatment of BGL may be performed prior to proceeding with the enrichment reaction.
References
  • Ganatra, M. B. et al. (2021). A bi‑specific lectin from the mushroom Boletopsis grisea and its application in glycoanalytical workflows. Sci. Rep. 11, 160.
  • Vainauskas, S. et al. (2016). Profiling of core fucosylated N-glycans using a novel bacterial lectin that specifically recognizes α1,6 fucosylated chitobiose. Sci. Rep. 6, 34195.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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