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β1-4 Galactosidase S

Catalog # Concentration Size List Price Quantity Your Price
P0745L 8000 units/ml 2000 units $883.00
$794.70
P0745S 8000 units/ml 400 units $220.00
$198.00
Catalog # Size List Price Your Price
P0745L 2000 units $883.00
$794.70
P0745S 400 units $220.00
$198.00
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

β1-4 Galactosidase S is a highly specific exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing β1-4 linked galactose residues from oligosaccharides.

  • Recombinant enzyme with no detectable endoglycosidase or other exoglycosidase contaminating activities
  • Glycerol-free for optimal performance in HPLC and mass spectrometry analysis
  • ≥95% purity, as determined by SDS-PAGE and intact ESI-MS
  • Optimal activity and stability for up to 24 months
β1-4 Galactosidase S is a highly specific exoglycosidase that catalyzes the hydrolysis of β1-4 linked galactose residues from oligosaccharides.

Specificity
Product Source
Cloned from Streptococcus pneumoniae and expressed in E. coli.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  β1-4 Galactosidase S P0745SVIAL -20 1 x 0.05 ml 8,000 units/ml
  GlycoBuffer 1 B1727SVIAL -20 1 x 1 ml 10 X
  β1-4 Galactosidase S P0745LVIAL -20 1 x 0.25 ml 8,000 units/ml
  GlycoBuffer 1 B1727SVIAL -20 1 x 1 ml 10 X

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to cleave > 95% of the terminal, β-D-galactose from 1 nmol Galβ1-4GlcNAcβ1- 3Galβ1-4Glc-7-amino-4-methyl-coumarin (AMC), in 1 hour at 37°C in a total reaction volume of 10 μl.

Reaction Conditions

1X GlycoBuffer 1
Incubate at 37°C

1X GlycoBuffer 1
5 mM CaCl2
50 mM sodium acetate
(pH 5.5 @ 25°C)

Storage Buffer

50 mM NaCl
20 mM Tris-HCl
1 mM EDTA
pH 7.5 @ 25°C

Heat Inactivation

65°C for 10 minutes

Molecular Weight

Apparent: 231000 daltons

Specific Activity

123,000 units/mg

Unit Assay Conditions

Two fold dilutions of β1-4 Galactosidase S are incubated with 1 nmol AMClabeled substrate and 1X GlycoBuffer 1 in a 10 μl reaction. The reaction mix is incubated at 37°C for 1 hour. Separation of reaction products are visualized via thin layer chromatography (1).

Companion Products
Notes
  • Reactions may be scaled-up linearly to accommodate larger reaction volumes.
  • The amount of exoglycosidase enzyme required varies when different substrates are used. Start with 1–2 μl for 1 μg of glycoprotein or 100 nM of oligosaccharide for one hour in a 10–25 μl reaction. If there is still undigested material, let the reaction go overnight.
References
  • Wong-Madden, S. T. and Landry, D. (1995). Glycobiology. 5, 19-28.
Web Tools
Citations
Publications
  • Albrecht, S., Vainauskas, S., Stöckmann, H., McManus, C., Taron, C.H. and Rudd, P.M. (2016). Comprehensive Profiling of Glycosphingolipid Glycans Using a Novel Broad Specificity Endoglycoceramidase in a High-Throughput Workflow. Anal Chem. May 3;88(9), 4795-802. Anal Chem..PubMedID: 27033327
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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