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O-Glycoprotease (IMPa)

Catalog # Concentration Size List Price Quantity Your Price
P0761S 1000 units/ml 200 rxns $1,130.00
$1,017.00
Catalog # Size List Price Your Price
P0761S 200 rxns $1,130.00
$1,017.00
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

O-Glycoprotease (IMPa) is a broad specificity protease that cleaves the peptide bonds of a glycoprotein or glycopeptide immediately N-terminal to a serine or threonine residue containing a mucin-type O-linked glycan with or without sialylation.

  • Enables O-glycosylation site mapping and O-glycan structural profiling
  • Efficiently cleaves adjacent to O-glycans with or without sialic acid, no neuraminidase treatment necessary
  • Recombinant enzyme with no detectable non-specific protease contaminating activities
  • Can be used under native or denaturing conditions
  • Glycerol-free for optimal performance in HPLC and mass spectrometry analysis
  • Supplied in solution; optimal activity and stability for up to 24 months
 

O-Glycoprotease (IMPa), also known as Immunomodulating protease (IMPa), is a broad specificity O-glycoprotease from Pseudomonas aeruginosa.  It recognizes mucin-type O-glycans in glycoproteins or glycopeptides, including branched and sialylated structures, and cleaves the peptide bond immediately N-terminal to a glycosylated serine or threonine residue. It can be used in glycoproteomics workflows to map O-glycosylated residues and structurally profile O-glycans present at each glycosite. O-Glycoprotease (IMPa) has a 6xHis-tag for easy removal from a reaction using nickel affinity resins.

 

Specificity:


Product Source
Cloned from Pseudomonas aeruginosa and expressed in E. coli.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  O-Glycoprotease (IMPa) P0761SVIAL -20 1 x 200 reactions 1,000 units/ml
Features
  • O-glycosylation site mapping
  • O-glycan structural determination
  • Enables characterization of simple and complex glycoproteins containing O-linked glycans
  • Efficiently cleaves glycoproteins with or without sialic acid, no neuraminidase treatment necessary
  • 200 reactions (one vial) is sufficient for the cleavage of up to 2 mg of glycoprotein
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Companion Products
Notes
  • O-Glycoprotease (IMPa) cleaves a variety of glycoproteins and glycopeptides containing mucin-type O-glycans with or without sialic acid, including Tn-antigen (GalNAc-α-Ser/Thr).
  • Glycoproteins can be sequentially digested with O-Glycoprotease (IMPa) followed by Trypsin-ultra Mass Spectrometry Grade (NEB #P8101).
  • The optimal reaction buffer for O-Glycoprotease (IMPa) activity is 20 mM Tris-HCl, pH 8.0. The optimal pH range for O-Glycoprotease (IMPa) is pH 7.0-8.0. Enzyme activity decreases significantly at pH values below 7.0 and above pH 8.0.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Change Notifications
Effective 9/27/2022, name modified to O-Glycoprotease (IMPa)
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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