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Lambda DNA (dam-)

Catalog # Concentration Size List Price Quantity Your Price
N3013L 500 µg/ml 1250 µg $451.00
$405.90
N3013S 500 µg/ml 250 µg $115.00
$103.50
Catalog # Size List Price Your Price
N3013L 1250 µg $451.00
$405.90
N3013S 250 µg $115.00
$103.50
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • Lambda DNA is a commonly used DNA substrate
  • Isolated from a dam- E.coli strain, this DNA can be digested with restriction endonucleases that are sensitive to dam methylation
  • View sequence details
Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7). The bacteriophage is grown in an E. coli host which is deficient in adenine methylase (dam-). Therefore, those deoxyadenosine positions which are methylated in a wild type E. coli host are not methylated in the dam- host. This DNA gives complete digests with restriction endonucleases that are sensitive to dam methylation. Lambda DNA (dam-) is 48,502 base pairs in length.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Lambda DNA (dam-) N3013SVIAL -20 1 x 0.5 ml 500 µg/ml
  Lambda DNA (dam-) N3013LVIAL -20 2 x 1.25 ml 500 µg/ml

Properties & Usage

Storage Buffer

10 mM Tris-HCl
1 mM EDTA
pH 8 @ 25°C



Companion Products
Notes
  • The advantage of this DNA is that it gives complete digests with those restriction endonucleases (BclI, MboI, DpnII, etc.) whose recognition sites are partially modified by most E. coli strains.
References
  • Daniels, D.L. et al. (1983). Appendix II: Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R.A. Weisberg(Ed.), Lambda-II. 519-676. New York: Cold Spring Harbor Laboratory Press.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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