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Histone H3.3 Human, Recombinant

Catalog # Concentration Size List Price Quantity Your Price
M2507S 1 mg/ml 100 µg
Please Inquire
Catalog # Size List Price Your Price
M2507S 100 µg
Please Inquire
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Histone H3.3, an H3 variant that is found in all eukaryotes from yeast to human, is replication and cell cycle phase-independent and is the most common H3 in non-dividing cells. It has been shown to be enriched in covalent modifications associated with gene activation (4,5).

  • Highly pure, recombinant protein
  • Identity confirmed using Edman Degradation to sequence intact protein; mass confirmed by ESI-TOF MS
  • Tested for exonuclease, endonuclease and protease contamination

Histone H3 combines with Histone H4 to form the H3/H4 tetramer. Two H2A/H2B heterodimers interact with an H3/H4 tetramer to form the histone octamer (1,2). It is also modified by various enzymes and can act as a substrate for them. These modifications have been shown to be important in gene regulation.

  

Protein Sequence: 
ARTKQ TARKS TGGKA PRKQL ATKAA RKSAP STGGV KKPHR YRPGT VALRE IRRYQ KSTEL LIRKL PFQRL VREIA QDFKT DLRFQ SAAIG ALQEA SEAYL VGLFE DTNLC AIHAK RVTIM PKDIQ LARRI RGERA (Genbank accession number: P84243)

ESI-TOF Mass Spectrometry Analysis of Histone H3.3 Human, Recombinant
ESI-TOF Analysis of Histone H3.3 Human, Recombinant


SDS-PAGE analysis of histone H3.3 Human, Recombinant
SDS-PAGE analysis of histone H3.3 Human, Recombinant
Lane 1 & 7: NEB Protein Ladder (NEB #P7703),
Lane 2 thru 6: 0.5, 1.0, 2.0, 5.0, 10.0 µg Histone H3.3 Human, Recombinant
Product Source
An E. coli strain that carries a plasmid encoding the cloned human histone H3.3 gene, H3F3A or H3F3B. (Genbank accession number: AK311905)

Properties & Usage

Storage Buffer

300 mM NaCl
1 mM EDTA
1 mM DTT
20 mM Sodium Phosphate
pH 7 @ 25°C

Molecular Weight

Theoretical: 15197 daltons


Companion Products
Notes
  • 1 mg/ml (66 μM) is calculated using the molar extinction coefficient for Histone H3.3 (3960) and its absorbance at 280  nm (6,7).  1.0 A280 units = 3.8 mg/ml.
  • Synonym: Histone H3.3A, H3F3, H3.3B.
References
  • Kornberg, R.D. (1989). Annu. Rev. Biochem.. 46, 931-954.
  • van Holde, K.E. (1989). Chromatin. 1-497,
  • Gabrielli et al. (1984). Mol. Cell. Biochem.. 65, 57-66.
  • Henikoff, S. et al. (2004). PNAS. 101, 1525-1530.
  • Hake, S.B. et al. (2006). J. Biol. Chem.. 281, 559-568.
  • Gill, S.C. and von Hippel, P.H. (1989). Anal. Biochem. 182, 319-326.
  • Pace, C.N. et al. (1995). Protein Science. 4, 2411-2423.
Quality Control Assay
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Specifications
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Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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