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Histone H3.1 Human, Recombinant

Catalog # Concentration Size List Price Quantity Your Price
M2503S 1 mg/ml 100 µg
Please Inquire
Catalog # Size List Price Your Price
M2503S 100 µg
Please Inquire
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Histone H3 combines with Histone H4 to form the H3/H4 tetramer. Histone H3.1 is an H3 variant that has thus far only been found in mammals, is replication dependent and is associated with gene activation and gene silencing.

  • Highly pure, recombinant protein
  • Mass confirmed by ESI-TOF MS
  • Tested for exonuclease, endonuclease and protease contamination

Histone H3 combines with Histone H4 to form the H3/H4 tetramer. Two H2A/H2B heterodimers interact with an H3/H4 tetramer to form the histone octamer. It is also modified by various enzymes and can act as a substrate for them. These modifications have been shown to be important in gene regulation.

Histone H3.1, an H3 variant that has thus far only been found in mammals, is replication dependent and is associated with gene activation and gene silencing.

Protein Sequence: ARTKQ TARKS TGGKA PRKQL ATKAA RKSAP ATGGV KKPHR YRPGT VALRE IRRYQ KSTEL LIRKL PFQRL VREIA QDFKT DLRFQ SSAVM ALQEA CEAYL VGLFE DTNLC AIHAK RVTIM PKDIQ LARRI RGERA (Genbank accession number: AAN10051)

SDS-PAGE analysis of Histone H3.1 Human, Recombinant
SDS-PAGE analysis of Histone H3.1 Human, Recombinant
Lane 1: NEB Unstained Protein Marker, Broad Range (NEB #P7702), Lane 2 thru 6: 0.5, 1.0, 2.0, 5.0, 10.0 µg Histone H3.1 Human, Recombinant.

 

ESI-TOF Mass Spectrometry Analysis of Histone H3.1 Human, Recombinant
ESI-TOF Analysis of Histone H3.1 Human, Recombinant
Product Source
An E.coli strain that carries a plasmid encoding the cloned human histone H3.1 gene, HIST1H3A or H3FA. (Genbank accession number: AF531274)

Properties & Usage

Storage Buffer

300 mM NaCl
1 mM EDTA
1 mM DTT
20 mM Sodium Phosphate
pH 7 @ 25°C

Molecular Weight

Theoretical: 15273 daltons


Companion Products
Notes
  • When running SDS-PAGE gels, Histone H3.1 can self-oligomerize if freshly prepared DTT (dithiothreitol) is not used when preparing the sample for gel electrophoresis.
  • 1 mg/ml, 65 µM is calculated using the molar extinction coefficient for Histone H3.1 (4080) and its absorbance at 280 nm (3,4). 1.0 A280 units = 3.8 mg/ml
References
  • Kornberg, R.D. (1977). Annu. Rev. Biochem. 46, 931-954.
  • van Holde, K.E. (1989). Chromatin. 1-497.
  • Gill, S.C. and von Hippel, P.H. (1989). Anal. Biochem.. 182, 319-326.
  • Pace, C.N. et al. (1995). Protein Science. 4, 2411-2423.
  • Hake, S.B. et al. (2006). J. Biol. Chem.. 281, 559-568.
Quality Control Assay
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Specifications
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Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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