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Histone H2B Human, Recombinant

Catalog # Concentration Size List Price Quantity Your Price
M2505S 1 mg/ml 100 µg
Please Inquire
Catalog # Size List Price Your Price
M2505S 100 µg
Please Inquire
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Histone H2B combines with Histone H2A to form the H2A-H2B heterodimer. Histone H2A is also modified by various enzymes and these modifications have been shown to be important in gene regulation.

  • Highly pure, recombinant protein
  • Mass confirmed by ESI-TOF MS
  • Tested for exonuclease, endonuclease and protease contamination

Histone H2B combines with Histone H2A to form the H2A-H2B heterodimer. Two H2A/H2B heterodimers interact with an H3/H4 tetramer to form the histone octamer. Histone H2B is also modified by various enzymes and can act as a substrate for them. These modifications have been shown to be important in gene regulation.

Protein Sequence: PEPAK SAPAP KKGSK KAVTK AQKKD GKKRK RSRKE SYSIY VYKVL KQVHP DTGIS SKAMG IMNSF VNDIF ERIAG EASRL AHYNK RSTIT SREIQ TAVRL LLPGE LAKHA VSEGT KAVTK YTSSK(Genbank accession number: AAN59961)

SDS-PAGE analysis of Histone H2B Human, Recombinant

Lane 1: NEB Unstained Protein Marker, Broad Range (NEB #P7702), Lane 2-6: 0.5 μg, 1.0 μg, 2.0 μg, 5.0 μg, 10.0 μg Histone H2B Human, Recombinant. 
ESI-TOF Mass Spectrometry Analysis of Histone H2B Human, Recombinant

Product Source
An E.coli strain that carries a plasmid encoding the cloned human histone H2B gene, HIST2H2BE or H2BFQ. (Genbank accession number: AY131979)

Properties & Usage

Storage Buffer

300 mM NaCl
1 mM EDTA
20 mM Sodium Phosphate
pH 7 @ 25°C

Molecular Weight

Theoretical: 13789 daltons


Companion Products
Notes
  • 1 mg/ml, 73 µM is calculated using the molar extinction coefficient for Histone H2B (6400) and its absorbance at 280 nm (3,4). 1.0 A280 units = 2.2 mg/ml
  • Synonyms: Histone H2B/q, Histone H2B.1,  Histone H2B-GL105
References
  • Kornberg, R.D. (1977). Annu. Rev. Biochem.. 46, 931-954.
  • van Holde, K.E. (1989). Chromatin. 1-497.
  • Gill, S.C. and von Hippel, P.H. (1988). Anal. Biochem.. 182, 319-326.
  • Pace, C.N. et al. (1995). Protein Science. 4, 2411-2423.
Quality Control Assay
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Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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