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Histone H4 Human, Recombinant

Catalog # Concentration Size List Price Quantity Your Price
M2504S 1 mg/ml 100 µg
Please Inquire
Catalog # Size List Price Your Price
M2504S 100 µg
Please Inquire
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Histone H4 combines with Histone H3 to form the H3/H4 tetramer. Histone H4 is also modified by various enzymes and these modifications have been shown to be important in gene regulation. 

  • Highly pure, recombinant protein
  • Mass confirmed by ESI-TOF MS
  • Tested for exonuclease, endonuclease and protease contamination
Histone H3 combines with Histone H4 to form the H3/H4 tetramer. Two H2A/H2B heterodimers interact with an H3/H4 tetramer to form the histone octamer (1,2). Histone H4 is also modified by various enzymes and can act as a substrate for them. These modifications have been shown to be important in gene regulation.

Protein Sequence:
SGRGK GGKGL GKGGA KRHRK VLRDN IQGIT KPAIR RLARR GGVKR ISGLI YEETR GVLKV FLENV IRDAV TYTEH AKRKT VTAMD VVYAL KRQGR TLYGF GG (Genbank accession number: AAM83108)

SDS-PAGE analysis of Histone H4 Human, Recombinant
SDS-PAGE analysis of Histone H4 Human, Recombinant
Lane 1 & 7: NEB Protein Ladder (NEB #P7703),
Lane 2 thru 6: 0.5, 1.0, 2.0, 5.0, 10.0 µg Histone H4 Human, Recombinant.

ESI-TOF Mass Spectrometry Analysis of Histone H4 Human, Recombinant
ESI-TOF Analysis of Histone H4 Human, Recombinant

Product Source
An E. coli strain that carries a plasmid encoding the cloned human histone H4 gene, HIST2H4. (Genbank accession number: AF525682)

Properties & Usage

Storage Buffer

300 mM NaCl
1 mM EDTA
20 mM Sodium Phosphate
pH 7 @ 25°C

Molecular Weight

Theoretical: 11236 daltons


Companion Products
Notes
  • The protein concentration (1 mg/ml, 89 µM) is calculated using the molar extinction coefficient for Histone H4 (5120) and its absorbance at 280 nm (3,4).  1.0 A280 units = 2.2 mg/ml.
  • Synonyms for HIST2H4 gene: H4/N, H4F2, H4FN
References
  • Kornberg, R.D. (1977). Annu. Rev. Biochem. 46, 931-954.
  • van Holde, K.E. (1989). Chromatin. 1-497,
  • Gill, S.C. and von Hippel, P.H. (1989). Anal. Biochem.. 182, 319-326.
  • Pace, C.N. et al. (1995). Protein Science. 4, 2411-2423.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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