New England Biolabs Canada
Expand menu
Sign In or Sign UpMy NEB

We are transitioning to our new website at www.neb.com, which will go live on April 7th, 2025.

On April 2nd at 8:00 PM ET, www.neb.ca e-commerce functionality will be disabled for the website transition. If you need to place your order between April 2nd at 8:00 pm ET to April 6th, then please call 1-800-387-1095 or email orders.ca@neb.com.

The transition to the new website will not affect our shipping schedule. If you're a registered user, all your account details, including order history and shipping details will be safely transferred to the new site.

Gel Loading Dye, Orange (6X)

Catalog # Concentration Size List Price Quantity Your Price
B7022S 6 X 4 ml $83.00
$74.70
Catalog # Size List Price Your Price
B7022S 4 ml $83.00
$74.70
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Gel Loading Dye, Orange (6X) is an Orange G-based loading dye for convenient gel loading and sharp bands

  • For use with agarose and non-denaturing polyacrylamide gels
  • Contains SDS for improved band sharpness
  • Contains EDTA to stop enzymatic reactions
  • Orange G will not appear in gel photographs
  • You may also be interested in our Gel Loading Dye, Purple (6X) with and without SDS, for sharp bands and no UV shadow.
Gel Loading Dye, Orange (6X) is a pre-mixed loading buffer with a tracking dye for agarose and non-denaturing polyacrylamide gel electrophoresis. This solution contains SDS, which often results in sharper bands, as some restriction enzymes are known to remain bound to their DNA substrates following cleavage. EDTA is also included to chelate magnesium (up to 10  mM) in enzymatic reactions, thereby stopping the reaction. Orange G will not appear in gel photographs, and runs ahead of all but the smallest restriction fragments. Orange G migrates at approximately 50 bp on a standard 1% TBE agarose gel. This product is packaged as 4x1 ml vials.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Gel Loading Dye, Orange (6X) B7022SVIAL 25 4 x 1 ml 6 X
Gel Loading Dye, Orange (6X) | New England Biolabs
Home Buffers Products Gel Loading Dye, Orange (6X)

Gel Loading Dye, Orange (6X)

Gel Loading Dye, Orange (6X) is an Orange G-based loading dye for convenient gel loading and sharp bands

  • For use with agarose and non-denaturing polyacrylamide gels
  • Contains SDS for improved band sharpness
  • Contains EDTA to stop enzymatic reactions
  • Orange G will not appear in gel photographs
  • You may also be interested in our Gel Loading Dye, Purple (6X) with and without SDS, for sharp bands and no UV shadow.
Blog_ContextualAd
Catalog https://www.neb.com/en/# Concentration Size
B7022S 6 X 4 ml
Please enter a quantity for at least one size

  • Product Information

    Gel Loading Dye, Orange (6X) is a pre-mixed loading buffer with a tracking dye for agarose and non-denaturing polyacrylamide gel electrophoresis. This solution contains SDS, which often results in sharper bands, as some restriction enzymes are known to remain bound to their DNA substrates following cleavage. EDTA is also included to chelate magnesium (up to 10  mM) in enzymatic reactions, thereby stopping the reaction. Orange G will not appear in gel photographs, and runs ahead of all but the smallest restriction fragments. Orange G migrates at approximately 50 bp on a standard 1% TBE agarose gel. This product is packaged as 4x1 ml vials.
    This product is related to the following categories:
    Gel Loading Buffers,
    Buffers Products

    • Reagents Supplied

      The following reagents are supplied with this product:

      NEB https://www.neb.com/en/# Component Name Component https://www.neb.com/en/# Stored at (°C) Amount Concentration

    • Properties & Usage

      1X Buffer Components

      2.5% Ficoll®-400
      11 mM EDTA
      3.3 mM Tris-HCl
      0.017% SDS
      0.15% Orange G
      pH 8@25°C

    • Product Notes

      1. Use 5 µl of Gel Loading Dye, Orange (6X) per 25 µl reaction, or 10 µl per 50 µl reaction. Mix well before loading gel.

  • Protocols, Manuals & Usage

  • FAQs & Troubleshooting

  • Citations & Technical Literature

    • Citations

      Product Citation Tool

  • Quality, Safety & Legal

    • Quality Control Assays

      Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

    • Specifications & Change Notifications

      The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    • Certificate of Analysis

      The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    • Safety Data Sheets

      The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
    • Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

      This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

      New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Ineligible item added to cart

Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly. If you have any questions, please contact Customer Support at freezers@neb.com or 1-800-632-5227 x 8.

Continue

Choose your country

If you don&https://www.neb.com/en/#39;t see your country above, please visit our international site

Loading Spinner

Session Expired

You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.

Institution Changed

Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.

Sign in to your NEB account

To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.

Sign In



Companion Products
Notes
  • Use 5 µl of Gel Loading Dye, Orange (6X) per 25 µl reaction, or 10 µl per 50 µl reaction. Mix well before loading gel.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Other Products You May Be Interested In
 Top