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3´-O-Me-m7G(5')ppp(5')G RNA Cap Structure Analog

Catalog # Concentration Size List Price Quantity Your Price
S1411L 5 µmol $986.00
$887.40
S1411S 1 µmol $248.00
$223.20
Catalog # Size List Price Your Price
S1411L 5 µmol $986.00
$887.40
S1411S 1 µmol $248.00
$223.20
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
For most RNAs the cap structure increases stability, decreases susceptibility to exonuclease degradation, and promotes the formation of mRNA initiation complexes.

  • Also known as Anti-Reverse Cap Analog (ARCA)
  • Produces 100% translatable capped transcripts
  • Can be used for co-transcriptional capping with T7 (NEB #M0251), SP6 (NEB #M0207) and T3 RNA Polymerases
  • Can be used for synthesis of m7G capped RNA for in vitro splicing assays
  • Can be used for synthesis of m7G capped RNA for transfection or microinjection
  • Supplied as a dried sodium salt
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    Avoiding RNase Contamination
Blocking of the 3´ -hydroxyl of m7G with 3´-0-Me assures that the capped transcripts are homogeneous. The 3´ - hydroxyl of the non-methylated G is the only 3´ - hydroxyl available for initiation (1).

The 5´terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level (2,3,4). For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation (5), and a decrease in the formation of the initiation complex of mRNAs for protein synthesis (5,6). Certain prokaryotic mRNAs containing a 5´ terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system (6). Also a cap requirement has been observed for splicing eukaryotic substrate RNAs (7).

A method using E. coli RNA Polymerase primed with m7G(5´)ppp(5´)G or m7G(5´)ppp(5´)A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas (8). Larger amounts of capped RNAs are produced by transcription systems using SP6 RNA Polymerase primed with m7G(5´)ppp(5´)G (7).
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  3´-O-Me-m7G(5')ppp(5')G RNA Cap Structure Analog S1411SVIAL -20 1 x 1 µmol
  3´-O-Me-m7G(5')ppp(5')G RNA Cap Structure Analog S1411SVIAL -20 5 x 1 µmol

Properties & Usage

Supplied As

Dried Sodium Salt

Suggested Working Concentration

10mM

Recommended Resuspension Volume

100 μl

Notes
  • Addition of 100 µl of water gives approximately a 10 mM solution.
References
  • Peng, Z.-H. et al. (2002). ORG.LETT.. 4(2), 161.
  • Shatkin, A. J. (1978). Cell. 9, 645-653.
  • Fillipowicz, W. (1978). FEBS Lett. 96, 1-11.
  • Banerjee, A.K. (1980). Microbiol. Rev.. 44, 175-205.
  • Miura, K. (1981). Adv. Biophys.. 14, 205-238.
  • Shatkin, A. J. et al. (1977). Nucleic Acids. Res.. 4, 3065-3081.
  • Konarska, M. M. et al. (1984). Cell. 38, 731-736.
  • Contreas, R. et al. (1982). Nucleic Acids. Res.. 10, 6353-6363.
  • Paterson, B. M. and Rosenberg, M. (1979). Nature. 279, 696-701.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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