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Trypsin-digested BSA MS Standard (CAM-modified)

Catalog # Concentration Size List Price Quantity Your Price
P8108S 500 pmol $138.00
$124.20
Catalog # Size List Price Your Price
P8108S 500 pmol $138.00
$124.20
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

This standard is a complex mixture of peptides produced by the tryptic digest of reduced and alkylated Bovine Serum Albumin (BSA).

  • Used to standardize MALDI-TOF or ESI mass spectrometers (TOF, Q-TOF, Ion Trap, or Orbitrap)
  • Standardization range of 500-2400 Da
  • Free of salts, glycerol and detergents
  • Supplied in dry format
  • Optimal stability for up to 24 months
A complex mixture of peptides produced by Trypsin digestion of Bovine Serum Albumin (BSA) that was reduced and alkylated with Iodoacetamide (CAM modified). This peptide mixture can be used to test a Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight (MALDI-TOF) or Electrospray Ionization (ESI) mass spectrometer (TOF, Q-TOF or Ion Trap).

MALDI Analysis of BSA Digest:
MALDI Analysis of BSA Digest:
The BSA digest is dissolved to 1 pmol/μl. One µl of digest is mixed with 1 µl of α-cyano-4-hydroxycinnamic acid (10 mg/ml in 50:50 acetonitrile:water with 0.1% trifluoroacetic acid). One μl of the digest/matrix solution were then spotted directly onto a MALDI target plate. 
Online Analysis of BSA Digest:
Online Analysis of BSA Digest:
The BSA digest solution was diluted to 100 fmol/μl with 0.1% formic acid. One μl (100 fmol) of the digest solution was injected via an Easy-nanoLC II (Proxeon) onto a self-packed reverse phase C18 nano column (Phenomenex packing material, 5 cm, 150 μm) and loaded onto a self-packed C18 analytical column with an integrated tip (New Objective Picofrit, 15 cm, 100 μm, Phenomenex packing material). Peptides were separated using a 45 min 5-70%B gradient (A = 0.1% formic acid, B = CH3CN, 0.1% formic acid) at a flow rate of 400 nl/min. Eluting peptides were analyzed online by a Q-Exactive mass spectrometer (Thermo Scientific) with an electrospray ion source. MS/MS spectra were acquired by HCD using an acquisition range of 400 to 1600 m/z.
Product Source
BSA (GENBANK P02769) was digested using Trypsin (TPCK-treated).
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Trypsin-digested BSA MS Standard (CAM-modified) P8108SVIAL -20 1 x 500 pmol

Properties & Usage



Notes
  • Supplied in dry format.
  • This product has lot-to-lot variations in peptide frequency and abundance.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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