Buffer Preparation Guidance (NEB #T1110)

Add supplied Monarch RNase A to Monarch Buffer B1 for a final concentration of 100 μg/ml. Mix and store at 4°C.

• For T1110G (10-prep) kit, add 12 µl of Monarch RNase A to Monarch Buffer B1.
• For T1110S (50-prep) kit, add 60 μl of Monarch RNase A to Monarch Buffer B1.
• For T1110L (250-prep) kit, add 285 μl of Monarch RNase A to Monarch Buffer B1.

Add isopropanol to the Monarch Buffer BZ prior to use (0.43 volumes of isopropanol per volume of Monarch Buffer BZ).

• For T1110G (10-prep) kit, add 1.5 ml of isopropanol to Monarch Buffer BZ.
• For T1110S (50-prep) kit, add 3.6 ml of isopropanol to Monarch Buffer BZ.
• For T1110L (250-prep) kit, add 18 ml of isopropanol to Monarch Buffer BZ.

Add ethanol to the Monarch Buffer WZ prior to use (4 volumes of ≥ 95% ethanol per volume of Monarch Buffer WZ).

• For T1110G (10-prep) kit, add 4 ml of ethanol to Monarch Buffer WZ.
• For T1110S (50-prep) kit, add 20 ml of ethanol to Monarch Buffer WZ.
• For T1110L (250-prep) kit, add 104 ml of ethanol to Monarch Buffer WZ.

Always keep all buffer bottles tightly closed when not in use.