Protocol for unwinding double stranded DNA with Tte UvrD Helicase (#M1202)

1. Prepare a 20 µl reaction as follows:
   
   

   Tte UvrD Helicase	20 ng (1 μL)
   DNA	up to 1 µg
   Isothermal Amplification Buffer (10X)	2 μL (1X)
   ATP (10 mM)	2 μL (1 mM)
   Nuclease-Free Water	to 20 μL
   Incubation Temperature	65°C
   Incubation Time	10 min

2. Incubate at 65°C for 10 minutes
   
   
3. Stop reaction by heating to 80°C or addition of EDTA (to 10 mM). 

Note: Unwound DNA will rapidly reanneal, so consider using a trapping or digestion approach to observe the unwound DNA product of the helicase reaction.