Protocol for Endonuclease IV (M0304)
1) Reaction set-up:
DNA | 10 pmol |
NEBuffer 3 (NEB #B7003) (10X) | 1-5 µl |
Endonuclease IV | 1 µl (10 units) |
H2O | Up to 10-50 µl |
2) Incubate at 37°C for 1 hour.
1) Reaction set-up:
DNA | 10 pmol |
NEBuffer 3 (NEB #B7003) (10X) | 1-5 µl |
Endonuclease IV | 1 µl (10 units) |
H2O | Up to 10-50 µl |
2) Incubate at 37°C for 1 hour.